Abstract: Objective:To investigate the expressions of villin,cytokeratin 7(CK7) and cytokeratin 20(CK20) in differentiating primary and intestinal tract metastatic liver tumors and evaluate the diagnostic value of combined markers including alpha-fetoprotein(AFP),hepatocyte paraffin 1(Hep-1),CK19 and carcinoembryonic antigen(CEA).Methods:The expressions of CK7,CK20 and villin were detected immunohistochemically with S-P method in 40 cases of hepatocellular carcinoma,35 cases of cholangiocarcinoma,40 cases of metastatic bowel cancer and 20 cases of normal liver tissue.Tumor cases were also marked with Hep-1,CK19,CEA and AFP.Results:Primary and metastatic liver tumors had the specific histological structure and morphology relatively.Combined these markers including CK7,CK20,villin,AFP,Hep-1,CK19 and can CEA could differentiate hepatocellular carcinoma and metastatic colorectal cancer,there was statistical difference(P0.05).CK7 and CK19 could differentiate primary intrahepatic bile duct cell carcinoma and metastatic colorectal cancer,there was statistical difference(P0.05).Conclusions:Better diagnosis and differential diagnosis can be made in primary and intestinal tract metastatic liver tumors based on the histological observation and comprehensive clinical analysis,with reference to the combined immune makers panel.