刘杜先, 许喆菲, 刘兰侠, 刘蕾蕾. 原发及肠道转移性肝癌中绒毛蛋白、细胞角蛋白7及20的表达研究[J]. 蚌埠医学院学报, 2012, 36(3): 301-303.
    引用本文: 刘杜先, 许喆菲, 刘兰侠, 刘蕾蕾. 原发及肠道转移性肝癌中绒毛蛋白、细胞角蛋白7及20的表达研究[J]. 蚌埠医学院学报, 2012, 36(3): 301-303.
    LIU Du-xian, XU Zhe-fei, LIU Lan-xia, LIU Lei-lei. Expressions of villin,cytokeratin 7,and cytokeratin 20 in primary and intestinal tract metastatic liver tumors[J]. Journal of Bengbu Medical College, 2012, 36(3): 301-303.
    Citation: LIU Du-xian, XU Zhe-fei, LIU Lan-xia, LIU Lei-lei. Expressions of villin,cytokeratin 7,and cytokeratin 20 in primary and intestinal tract metastatic liver tumors[J]. Journal of Bengbu Medical College, 2012, 36(3): 301-303.

    原发及肠道转移性肝癌中绒毛蛋白、细胞角蛋白7及20的表达研究

    Expressions of villin,cytokeratin 7,and cytokeratin 20 in primary and intestinal tract metastatic liver tumors

    • 摘要: 目的:检测原发及肠道转移性肝脏肿瘤中绒毛蛋白(villin)、细胞角蛋白7及20(CK7、CK20)的表达情况,评测联合标记甲胎蛋白(AFP)、Hep-1、CK19、癌胚抗原(CEA)对肝肿瘤的诊断价值。方法:采用免疫组织化学S-P法检测原发性肝癌40例,胆管癌35例,肠道转移性肝癌40例,正常肝组织20例中villin、CK7和CK20的表达。3组肿瘤联合标志AFP、Hep-1、CK19、CEA。结果:原发性肝癌及肠道转移性肝癌组织学存在相对特异的结构和形态,在组织学基础上联合检测CK7、CK20、villin、AFP、Hep-1、CEA对原发性肝癌与肠道转移性肝癌间的鉴别诊断价值高,差异有统计学意义(P;0.05)。CK7、CK19对胆管癌与肠道转移性肝癌间的鉴别价值差异有统计学意义(P;0.05)。结论:以HE光镜组织学为基础,参考联合免疫标志结果,结合临床综合分析,可以对原发性肝癌和肠道转移性肝癌进行较好的诊断和鉴别诊断。

       

      Abstract: Objective:To investigate the expressions of villin,cytokeratin 7(CK7) and cytokeratin 20(CK20) in differentiating primary and intestinal tract metastatic liver tumors and evaluate the diagnostic value of combined markers including alpha-fetoprotein(AFP),hepatocyte paraffin 1(Hep-1),CK19 and carcinoembryonic antigen(CEA).Methods:The expressions of CK7,CK20 and villin were detected immunohistochemically with S-P method in 40 cases of hepatocellular carcinoma,35 cases of cholangiocarcinoma,40 cases of metastatic bowel cancer and 20 cases of normal liver tissue.Tumor cases were also marked with Hep-1,CK19,CEA and AFP.Results:Primary and metastatic liver tumors had the specific histological structure and morphology relatively.Combined these markers including CK7,CK20,villin,AFP,Hep-1,CK19 and can CEA could differentiate hepatocellular carcinoma and metastatic colorectal cancer,there was statistical difference(P0.05).CK7 and CK19 could differentiate primary intrahepatic bile duct cell carcinoma and metastatic colorectal cancer,there was statistical difference(P0.05).Conclusions:Better diagnosis and differential diagnosis can be made in primary and intestinal tract metastatic liver tumors based on the histological observation and comprehensive clinical analysis,with reference to the combined immune makers panel.

       

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