Abstract: Objective:To study the medical students' subjective well-being and the influencing factors. Methods:Two hundred medical students were inquired by the general well-being(GWB) and AFW-US. Results:GWB of the medical students was significantly higher than that of the national norm(P0.01). The difference in GWB was significant among students from different residences(P0.05),but not between different genders or being the only-child or not(P0. 05). There were significant differences in self concept among students of different genders, from the urban or rural areas and being the only-child or not (P 0. 05 to P 0. 01);Family environment factors also affected the GWB of students from urban or rural areas and of being the only-child or not(P0.05 to P0.01);different genders showed significant differences in job and social relation adaptation(P0.01). The subjective well-being of medical students was positive related to the self concept, family environment, job and social relation, amativeness, interpersonal interaction, school life and recreation activities(P 0. 05 to P 0. 01). Regression analysis showed that self concept and school life could significantly forecast the students' subjective well-being. Conclusions:Medical students display a relatively high level of general well-being, and self concept and school life are important factors affecting their subjective well-being.