Clinical application of the UF-500i Flow Cytometer in screening the asymptomatic urinary infection of pregnant women
-
摘要: 目的:采用UF-500i型尿流式分析仪检测妊娠妇女中段尿,以中段尿细菌培养为金标准,评价UF-500i型尿流式分析仪在筛查妊娠妇女无症状泌尿道感染(UTI)中的价值。方法:对500份妊娠妇女中段尿标本做细菌培养菌落计数,并与UF-500i型尿流式分析仪结果进行比较。结果:UF-500i型尿流式分析仪阳性检出率为10.4%,细菌培养阳性检出率为8.6%,差异无统计学意义(P0.05),且一致性较好(Kappa=0.999,P0.05)。UF-500i型尿流式分析仪筛选UTI的敏感度和特异性分别为88.4%和96.9%,阳性预测值和阴性预测值分别为73.1%和98.9%。结论:UF-500i型尿流式分析仪筛查妊娠无症状UTI具有快速、方便、经济的特点,有很高的实用价值,但要注意尿液污染问题。Abstract: Objective:To evaluate the clinical application of the UF-500i Flow Cytometer in screening the asymptomatic urinary infection of pregnant women.Methods:The leukocytes and bacteria in 500 mid-stream urine specimens of pregnant women were detected and compared with UF-500i.Results:The positive rates of UF-500i and urine culture were 10.4% and 7.6% respectively,and their differences had no statistical significance and kept considerable consistency(Kappa=0.999,P》0.05).The sensitivity and specificity of the UF-500i detection for urinary infection were 88.4% and 96.9% and the positive and negative predictive value were 73.1% and 98.9% respectively.Conclusions:The UF-500i Flow Cytometer in screening the asymptomatic urinary infection of pregnant women is rapidly,convenient and economical,which has good application value,and the urine contamination must be pay attention to.
-
Keywords:
- urinary infection /
- pregnancy complications /
- flow cytometer /
- urine cuture /
- urine contamination
-
-
[1] 丰有吉，沈铿.妇产科学[M].2版.北京:人民卫生出版社，2010:156-157 [1] [2] 陈颧珠.实用内科学[M] . 12版.北京:人民卫生出版社，2005:2217-2222 [2] [3] 叶应妩，王毓三，中子瑜.全国临床检验操作规程[M] . 3版.南京:东南大学出版社，2006:744 [3] [4] Terajima S, Yokomizo H, Yagi A, et al. L,valuation study forreference intervals of urine sediments using UF-1000i in medicalcheckup population[J].Sysmex J,2008,31:82-86.
[4] [5] Gross S, Bruschetta G, De Rosa R, et al. lmproving the efficiencyand efficacy of pre-analytical and analytical work-flow of urinecultures with urinary flow cytometry [J].New Microbiol, 2008,31 (4):501-505.
[5] [6] Wang,J, Zhang Y, Xu D, et al. L,valuation of the sysmex UF-1000ifor the diagnosis of urinary tract infection[J].Am J Clin Pathol,2010,133 (4):577-582.
[6] [7] 吴玉竹，邓莉.前、中段尿对UF-100尿沉渣分析仪在女性患者尿沉渣检测中的应用[J].郧阳医学院学报，2005,24 (5):36. [7] [8] 管琳，沈维维，雄萍.UF-2100尿沉渣分析仪在女性患者尿沉渣分析中的应用[J].检验医学，2007,4 (22):494. [8] [9] 孟静，周觅，王慧.尿沉渣UF-100检查中鉴别女性尿路感染与尿液污染指标的探讨[J].中国实验诊断学，2011,15(1):131-133.
计量
- 文章访问数: 3279
- HTML全文浏览量: 372
- PDF下载量: 212
下载:
