-
摘要: 正弓形虫是一种广泛存在于人体和动物中能引起人兽共患疾病的原虫，呈世界性分布。由于其感染率高，临床症状复杂，流行广泛，对人和畜牧业造成严重的威胁，越来越受到人们的重视。弓形虫生活史分为速殖子、缓殖子、裂殖体、配子体和卵囊5个时期。其中，速殖子与缓殖子是其主要致病阶段。急性感染期的虫体生长繁殖迅速被称为速殖子，慢性感染期的虫体生长繁殖缓慢被称为缓殖子，缓殖子在宿主免疫功能低。
-
-
[1] 《弓形虫膜抗原SAG3基因打靶载体构建方法探讨》[J].热带医学杂志,2004(3).＜br＞ [1] [2] 《弓形虫膜抗原SAG3基因打靶载体的构建及筛选》[J].中国人兽共患病杂志,2004(12).＜br＞ [2] [3] 《刚地弓形虫RH株微线体蛋白MIC3成熟肽的克隆与表达》[J].中国人兽共患病杂志,2005(4).＜br＞ [3] [4] 《弓形虫GRA4基因真核表达重组质粒DNA免疫小鼠的研究》[J].中国寄生虫病防治杂志,2005(3).＜br＞ [4] [5] 《弓形虫GRA4基因真核表达重组质粒的构建与表达》[J].中国人兽共患病杂志,2005(9).＜br＞ [5] [6] 《弓形虫表面抗原和致密颗粒蛋白的研究进展》[J].中国人兽共患病学报,2007(3).＜br＞ [6] [7] Crawford J,Grujic O,Bruic E,et al.Structural characterization of the bradyzoite surface antigen(BSR4)from Toxoplasma gondii,a unique addition to the surface antigen glycoprotein1-related superfamily[J].Biol Chem,2009,284(14):9192-9198.＜br＞
[7] [8] Kim SK,Karasov A,Boothroyd JC.Bradyzoite-specific surface antigen SRS9plays a role in maintaining Toxoplasma gondii persistence in the brain and in Host control of parasite replication in the intestine[J].Infect Immun,2007,75(4):1626-1634.＜br＞
[8] [9] Ferragut C,Soete M,Engels A,et al.Molecular cloning of the Toxoplasma gondii sag4gene encoding an18kDa bradyzoite specific surface protein[J].Mol Biochem Parasitol,1996,82(1):237-244.＜br＞
[9] [10] Bohne W,Gross U,Ferguson DJP,et al.Cloning and characterization of a bradyzoite-specifically expressed gene(hsp30/bag1)of Toxoplasma gondii,related to genes encoding small heat-shock proteins of plants[J].Mol Microbiol,1995,16(6):1221-1230.＜br＞
[10] [11] Nakaar V,Samuel BU,Ngo EO,et al.Targeted reduction of nucleoside triphosphate hydrolase by antisense RNA inhibits Toxoplasma gondii proliferation[J].Biol Chem,1999,274(8):5083-5087.＜br＞
[11] [12] Delauw MF,Guyt B,Torpier G,et al.Molecular characterization of a23-kilodalton major antigen secreted by Toxoplasma gondii[J].Immunology,1989,86(1):7537-7541.
[12] [13] [13] Mercier C,Howe DK,Mordue D,et al.Targeted disruption of the GRA2locus in Toxoplasma gondii decreases acute virulence in mice[J].Infect Immun,1998,66(9):4176-4182.
[14] [15] [14] Chen R,Lu SH,Tong QB,et al.Protective effect of DNA-mediated immunization with liposome-encapsulated GRA4against infection of Toxoplasma gondii[J].J Zhejiang Univ Scie B,2009,10(7):512-521.
[16] [17] [15] Carruthers VB,Giddings OK,Sibley LD.Secretion of micronemal proteins is associated with toxoplasma invasion of host cells[J].Cell Microbiol,1999,1(3):225-235.
[18] [19] [16] He XL,Grigg ME,Boothroyd JC,et al.Structure of the immunodominant surface antigen from the Toxoplasma gondii SRS superfamily[J].Nat Struct Biol,2002,9(8):606-611.
[20] [21] [17] Savva D,Morris JC,Johnson J.D,et al.Polymerase chain reactionfor detection of Toxoplasma gondii[J].J Med Microbiol,1990,32(1):25-31.
[22] [23] [18] Harning D,Spenter J,Metsis A,et al.Recombinant Toxoplasma gondii surface antigen1(P30)expressed in escherichia coli is recognized by human Toxoplasma-specific immunoglobulin M and IgG antibodies[J].Clin Diag Lab Immun,1996,3(3):355-357.
[24] [25] [19] 田小东.弓形虫SAGI、MIC3单基因及SAGI-MIC3融合基因真核表达载体的构建与鉴定[D].济南:山东大学,2008. [26] [27] [20] Lekutis C,Ferguson,Boothroyd JC.Toxoplasma gondii:identification of a developmentally regulated family of genes related to SAG2[J].Exp Parasitol,2000,96(1):89-96.
[28] [29] [21] Huang XH,Xuan XN,Suzuki H,et al.Characterization of Toxoplasma gondii SAG2expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay[J].Clin Diag Lab Immun,2002,9(6):1343-1347.
[30] [31] [22] Machado AV,Caetanoa BC,Barbosa RP,et al.Prime and boost immunization with influenza and adenovirus encoding the Toxoplasma gondii surface antigen2induces strong protective immunity[J].Vaccine,2010,28(1):3247-3256.
[32] [33] [23] 张玉英.弓形虫表面抗原SAG3在虫体入侵及增殖中的作用[D].广州:广东药学院,2009. [34] [24] Dzierszinski F,Mortuaire M,Delauw MF,et al.Targeted disruption of the glycosylphosphatidylinositolanchored surface antigen SAG3 gene in Toxoplasma gondii decreases host cell adhesion and drastically reduces virulence in mice[J].Mol Microbiol,2000,37(3):574-582.
[35] [36] [25] Knoll LJ,Boothroyd JC.Isolation of developmentally regulated genes from Toxoplasma gondii by a gene trap with the positive and negative selectable marker hypoxanthine-xanthine-guanine phosphoribosyltransferase[J].Mol Cell Biol,1998,18(2):807-814.
[37] [38] [26] 《弓形虫缓殖子期特异性抗原1基因的克隆、表达及对重组抗原的免疫反应性分析》[J].中国寄生虫学与寄生虫病杂志,2007(4). [39] [40] [27] 《弓形虫缓殖子期特异抗原SAG2C基因的克隆、鉴定与生物信息学分析》[J].热带医学杂志,2008(3). [41] [42] [28] 《弓形虫棒状体蛋白的研究进展》[J].中国兽医科学,2008(5). [43] [44] [29] 《弓形虫SAG3基因克隆及生物信息学分析》[J].中国病原生物学杂志,2008(6). [45] [46] [30] 《弓形虫微线体蛋白MIC3基因在大肠杆菌内的高效表达研究》[J].安徽农业科学,2008(17). [47] [48] [31] 《刚地弓形虫GJS株微线体蛋白3基因真核表达质粒的构建及在BHK-21细胞中的表达》[J].中国兽医科学,2009(2). [49] [50] [32] 《刚地弓形虫SAG3基因体外扩增及生物信息学分析》[J].热带医学杂志,2009(5). [51] [52] [33] 《弓形虫GJS株表面抗原1基因的原核细胞表达及其抗原性分析》[J].中国人兽共患病学报,2009(8). [53] [54] [34] 《弓形虫表面抗原SAG4基因的克隆、表达和鉴定》[J].中国寄生虫学与寄生虫病杂志,2009(4). [55] [56] [35] 《弓形虫GJS株pcDNA3-MIC3核酸疫苗的研究》[J].中国兽医学报,2009(10). [57] [58] [36] 《弓形虫主要表膜P30抗原的免疫保护作用研究》[J].泰山医学院学报,2001(1). [59] [60] [37] 《弓形虫微线体蛋白1部分基因原核表达重组质粒pWR4501MIC1的构建、鉴定及测序》[J].南华大学学报：医学版,2002(2). [61] [62] [38] 《弓形虫P24基因编码蛋白的分子生物学研究进展》[J].国外医学：寄生虫病分册,2004(3).
计量
- 文章访问数: 3244
- HTML全文浏览量: 317
- PDF下载量: 143
下载:
