L-选择素在肝癌细胞株HepG2中的表达

    Expression of L-selectin in HepG2 cell line of hepatocellular carcinoma

    • 摘要: 目的:观察L-选择素在人正常肝细胞株(L-02)和人肝癌细胞株(HepG2)中的表达。方法:应用RT-PCR法检测肝癌细胞株HepG2、正常肝细胞株L-02中L-选择素mRNA表达情况;应用流式细胞术检测HepG2细胞、L-02细胞中L-选择素的蛋白表达。结果:L-选择素mRNA在HepG2细胞中有表达,而在正常肝细胞株L-02细胞中无表达;HepG2细胞L-选择素的表达量为(10.090.51)%,而在L-02细胞中则无表达。结论:L-选择素在HepG2细胞中有表达而在L-02细胞中不表达。

       

      Abstract: To observe the expression of Lelectin in human hepatocellular caroinoma cell line(HepG2)and humannormal liver cell line(L-02). Methods:The gene mRNA and protein expression levels of Lelectin in HepG2 and L-02 were detectedby RTCR and flow cytometry respectively. Results: The mRNA expression of Lelectin gene was found in HepG2 cells but not in L-02 cells the protein expression of Lelectin in HepG2 cells was(10. 09士0. 51)%,but not in L-2 cells. Conclusions:In gene andprotein levels Lelectin can express in HepG2 but not in L-2

       

    /

    返回文章
    返回