曲安奈德对核因子-B、血管内皮生长因子在大鼠视网膜新生血管中表达的影响

秦梅; 岳晓丽; 方丽; 郁佳; 亓晓燕; 杨洪霞; 周琦

曲安奈德对核因子-B、血管内皮生长因子在大鼠视网膜新生血管中表达的影响

The effect of triamcinolone acetonide on nuclear factor-KB andvascular endothelial growth factor expression in retinal neovascularization in rats

摘要

摘要: 目的：探讨曲安奈德对核因子-B(NF-B)及血管内皮生长因子(VEGF)在大鼠视网膜新生血管病变中表达的影响。方法：选择7dSD幼鼠40只，其中30只大鼠建立氧诱导的视网膜新生血管病变模型，10只置于正常环境中。30只大鼠中20只大鼠出氧箱后给予玻璃体腔内注射曲安奈德1l(0.04mg)，对侧眼注射平衡盐溶液(balancedsaltsolution，BSS)作为对照，分为高氧+药物治疗组和高氧+BSS液对照组，每组20只眼；另10只大鼠作为高氧对照组。视网膜组织切片HE染色，免疫组织化学法检测NF-B及VEGF的表达。结果：高氧+药物治疗组与高氧对照组及高氧+BSS组相比，新生血管内皮细胞核数目明显减少(p0.01)，高氧+药物治疗组的NF-B及VEGF的表达均低于高氧对照组及高氧+BSS组(p0.05)。VEGF表达升高的同时，NF-B的表达呈升高趋势。结论：曲安奈德明显抑制NF-B及VEGF的表达，即有抑制新生血管形成的作用，NF-B与VEGF的表达呈正相关，在新生血管形成过程中起协同作用。

Abstract: To investigate the effect of triamoinolone acetonide on the nuclear factorcB(NFcB)and vascular endothelialgrowth factor(VEGF)expression in retinal neovascularization of rats. Methods:Forty SD 7 days old rats were selected 10 rats were innormal environment as control. Models of retinal neovascularization of 30 rats were established using oxygennduced which weredivided into other three groups 10 rats cavum vitreum were injected with 1 p,1(0. 04 mg)of triamoinolone acetonide as hyperoxia. ombined with medication group 10 rats cavum vitreum were injected with balanced saltsolution(BSS)as hyperoxia combined with BSSgroup and the left 10 rats acted as hyperoxia control group. HE staining the expression of NFcB and VEGF using immunohistochemicalmethod in the retina of 20 eyes of each group were explored. Results: The number of neovascular nuclei of the rat retina of hyperoxia. ombined with medication group were significantly less than that of hyperoxia control group and hyperoxia combined with BSS group(p0. O1). However the expression of NFcB and VEGF were significantly lower(p0. OS). With the high level of expression ofVEGF the expression of NFcB in the retina was also rising. Conclusions:Triamcinolone plays a role in inhibiting retinalneovascularization and the expression of NFycB and VEGFand the expression of NFycB and VEGF in the retina showes positive correlation

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