三氧化二砷对HL-60细胞端粒酶hTERT启动子活性的影响

Effect of arsenic trioxide on telomerase hTERT promoter activity in HL-60 cells

摘要: 目的:探讨三氧化二砷(As2O3)抑制HL-60细胞端粒酶hTERT表达的分子机制。方法:构建含有hTERT启动子不同片段的荧光素酶基因表达载体,转染HL-60细胞,分别在有无As2O3作用下检测各组荧光素酶活性变化,间接反映As2O3对hTERT启动子不同片段活性的影响。结果:在As2O3作用下,含有hTERT启动子不同片段的荧光素酶基因表达载体的荧光素酶活性均下降(P0.05～P0.01)。结论:As2O3对HL-60细胞端粒酶hTERT启动子活性具有抑制作用。

Abstract: Objective：To investigate the molecular mechanism of arsenic trioxide (As2O3)inhibiting telomerase human telomerasereverse transcriptase(hTERT) expression in HL-60 cells. Methods：The different fragments of hTERT promoter luoiferase geneexpression vector were construc;ted and transfected into HL-60 cells with or without As2O3. The changes of luoiferase activity whichindirectly rellected the role of As2O3 on hTERT promoter different fragments were detected. Results：The luoiferase activities in differentfragments of hTERT promoter gene expression vector were decreased with As2O3(P0.05toP0.01)Conclusions：As2O3 can inhibit telomerase activity of hTERT promoter in HL-60 cells