Abstract: Objective: To investigate the trend of hepatitis B virus (HBV) infection in students enrolled in Bengbu Medical College in 2009 in order to provide scientific evidence for preventing and controlling HBV happening.Methods: Hepatitis B surface antigen (HBsAg),alanine aminotransferase (ALT) and total bilirubin (TBIL) levels of fasting blood plasma form 3120 students enrolled in Bengbu Medical College in 2009 were detected by enzyme-linked immunoadsordent assay,improved Reitman method and diazo-reaction method respectively.Results: In 3120 freshmen,there were 99 students who determined with positive HBsAg expression,the HBsAg infection rate was 3.17%;but there was no statistical difference (P>0.05) between the rate of male freshmen HBsAg infection (3.77%) and female freshmen HBsAg infection (2.82%).The abnormal rate of ALT and TBIL concentrations in male freshmen had statistical difference (P<0.01) compared with their abnormal rate of female freshmen,but there was no statistical difference (P>0.05) between the rate of HBsAg infection in freshmen who detected with abnormal ALT and others who detected with abnormal TBIL.Conclusions: The rate of HBsAg infection in the freshmen of our college is much lower than that of the social crowd,and it is necessary to strengthen management of students who carried hepatitis B virus,inoculate hepatitis B vaccine for susceptible population,and prevent the spread of hepatitis B virus in college.