2种方法检测乙型肝炎表面抗原的比较
Comparison of two methods of detecting serum hepatitis B surface antigen
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摘要: 目的:比较金标法(GICA)与酶联免疫吸附法(ELISA)检测乙型肝炎表面抗原(HBsAg)的结果。方法:对5632份血清标本同时采用GICA和ELISA检测HBsAg,以ELISA为标准,对GICA的灵敏度、特异性进行评价。结果:5632份血清中以ELISA为标准,HBsAg阳性507例,阳性率为9.00%;GICA有34份假阴性,31份假阳性,灵敏度为93.84%,特异性为99.34%。34例假阴性中HBsAg均为弱阳性OD/CO值在1.50~4.76。2种方法测定的HBsAg结果差异无统计学意义(P0.05),且具有高度一致性(Kappa=0.9294,p0.01)。结论:GICA适用于单份检测,无需任何仪器,且快速简便,但灵敏度、特异性稍差,特别是针对HBsAg弱阳性有漏诊可能;ELISA虽然步骤多,时间长,但灵敏度高,特异性强,适合批量检测。实验室应根据实际需要结合工作情况选用。Abstract: To compare the detecting hepatitis B surface antigen(HBsAg)results by GICA and ELISA. Methods:The serum HBsAg of 5632 cases were detected by GICA and ELISA methods the sensitivity and specificity of GICA was evaluatedaccording to ELISA standard. Results:Based 9.00% by GICA.