Abstract: Objective:To investigate the drug resistance of Klebsiellu pueumouiue isolated from different infection positions and guide the clinical rational medication.Methods:From Jan.2010 to Sep.2011,the extended-sprctrum -lactamases(ESBLs) of 260 strains of Klebsiellu pueumouiue isolated from different infection positions were detected using ATB EXPRESSION semi-automatic microorganism analyzer according to the standard of the clinical and Laboratory Standards Association recommending diagnostic tests.Results:The detection rates of ESBLs and drug resistance to ciprofloxacin,cefotaxime,cefuroxime,imipenem,meropenem,piperacillin and ticarcillin/clavulanate of Klebsiellu pueumouiue isolated from 2011 year were higher than that from 2010 year(P0.05 to P0.01).The drug resistance to cefotaxime,piperacillin and tazobactam of Klebsiella pneumoniae isolated from infection position was lower than that from non-infection position(P0.05).There were not significant difference between the detection rate of ESBLs and drug resistance to 20 kinds of antibiotics in the older group and young adults group(P0.05).Conclusions:The drug resistance has different characteristics in different infection postions.The antibacterial drug should be reasonably used according to different characteristics in clinic,which can delay the emergence of drug resistance of bacteria.