皖北地区乙型肝炎患者HBV血清标志物与HBV-DNA定量检测的比较
Comparison of HBV-M and HBV-DNA detection in HBV infectors in the North of Anhui
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摘要: 目的:比较乙型肝炎病毒血清标志物(HBV-M)与HBV-DNA定量检测的结果。方法:采用酶联免疫法检测388例患者血清HBV-M,同时用荧光定量-聚合酶链反应检测其HBV-DNA含量。结果:乙型肝炎大三阳组患者血清HBV-DNA阳性率为91.25%,阳性患者中HBV拷贝数为107 IU/ml的所占比例最多,小三阳组阳性患者中HBV拷贝数以103 IU/ml为主,大三阳组患者血清HBV-DNA阳性率高于小三阳组(P0.05);HBsAg(+)、HBeAg()、抗-HBc(+)组阳性率为66.67%;HBsAg(+)、HBeAg(+)、抗HBc(-)组阳性患者中以105 IU/ml和106 IU/ml为主;HBsAg(+)、HBeAg(-)、HBcAb(+)组阳性率为30.77%。结论:乙型肝炎患者中HBeAg(+)者病毒复制水平最高,其与HBV-DNA密切相关;抗-HBe(+)、抗-HBc(+)患者病毒复制并非完全停止,但其复制水平明显降低;联合检测血清HBV-M与HBV-DNA可反映乙型肝炎患者免疫反应状态和病毒复制水平,为临床监测病情和用药提供实验依据。Abstract: Objective:To compare the detection results of HBV-M and HBV-DNA.Methods:All 388 HBV patients were detected for the HBV-M by ELISA and HBV-DNA by FQ-PCR.Results:The positive rate of HBV-DNA in the group of HBsAg(+)/HBeAg(+)/HBcAb(+) was 91.25%,and the majority of the virus replication was 107 IU/ml;while the majority of the virus replication in the group of HBsAg(+)/HBeAb(+)/ HBcAb(+) was 103 IU/ml.The positive rate of HBV-DNA in the group of HBsAgAb(+)/HBeAg(+)/HBc(+) was higher than that in the group of HBsAg(+)/HBeAb(+)/HBcAb(+)(P0.05).The positive rate of HBV-DNA in the group of HBsAg(+)/HBeAg()/ HBc(+)Ab was 66.67%.The majority of the virus replication was 105 IU/ml and 106 IU/ml in patients with positive HBsAg(+)/HBeAg(+)/HBc(-)Ab.The positive rate of HBV-DNA in the group of HBsAg(+)/HBeAg(-)/HBcAb(+) was 30.77%.Conclusions:Patients with HBeAg(+) have the highest replication level in all the groups,which is closely associated with HBV-DNA.Virus replication in patients with HBeAb(+) and HBcAb(+) does not stop completely,but obviously decreases.Combination detection of HBV-M and HBV-DNA may display the immune status and virus replication level of patients with hepatitis B,which would provide experimental basis for clinical monitoring and drug administration.
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