肺癌组织survivin mRNA的表达及其与预后的关系

    Expression of survivin mRNA in lung cancer tissue and its relationship with prognosis

    • 摘要: 目的: 探讨生存素(survivin)mRNA在肺癌组织中的表达与临床病理及其预后的关系。方法: 应用逆转录聚合酶链反应(RT-PCR)检测41例肺癌患者、9例良性肺疾病患者手术标本中的survivin mRNA表达,并随访2年,将其表达及表达强度与临床病理特征进行相关分析。结果: 肺癌患者手术标本癌组织中survivin mRNA表达的阳性率(70.7%)均高于癌旁正常组织(17.1%)和良性肺疾病患者组织survivn mRNA表达的阳性率(1/9)(P<0.005);癌旁正常组织与良性肺疾病组织survivin mRNA表达的阳性率差异无显著性(P>0.05);survivin mRNA在肺癌组织的表达及表达强度与年龄、性别、病理分型、病理分级、解剖部位分型及淋巴结和(或)不同肺叶及远处转移均无明显关系(P>0.05),但与预后有明显关系(P=0.001 8)。结论: 肺癌组织检测survivin mRNA表达可以作为肺癌诊断的特异性分子标志物之一,且可独立作为预测肿瘤的预后。

       

      Abstract: Objective: To assess the value of detection of survivin mRNA in lung cancer tissues.Methods: The specimens and the para-carcinomatous normal tissues of 41 patients with lung cancer and 9 patients with benign pulmonary diseases were collected.Reverse transcriptase polymerase chain reaction(RT-PCR) was performed to detect the survivin mRNA expression in the specimens.All the patients were followed-up for 2 years.The results were analyzed relatively.Results: The positive rate of survivin mRNA in the carcinomatous tissues(70.7%) was higher than that in the para-carcinomatous normal tissues(17.1%) and the benign pulmonary diseases tissues(1/9)(P<0.005).There was no significant difference between the para-carcinomatous normal tissues and the benign pulmonary diseases tissues(P>0.05).survivin mRNA expression was not related to the age,sex,histological subtype,differentiation,pathologic grading or lymph node metastases.Conclusions: Expression of survivin mRNA in the lung cancer tissue can be used as a specific molecular marker for the diagnosis of lung cancer.It can act as an independent prognostic factor.

       

    /

    返回文章
    返回