血铅检测实验条件规范化的探索

    Study on establishment of standardized measuring method for blood lead

    • 摘要: 目的:建立规范化的血铅检测实验条件。方法:血铅浓度的检测采用钨舟无焰原子吸收光谱法。按照NCCLS及卫生部血铅检测技术规范的要求,进行不精密度、不准确度、方法检测线、定量检测线、可报告范围、交叉污染率等性能评价。结果:血铅含量与吸光度值的相关系数r=0.999 9(t=103.06,P<0.01),不精密度、不准确度、方法检测线、定量检测线符合要求,血铅浓度在<298μg/L的范围内可准确报告定量结果,平均交叉污染率为0.378%~0.394%。结论:建立的实验条件稳定、可重复,具有临床推广价值。

       

      Abstract: Objective: To establish standardized measuring method for blood lead.Methods: Tungsren-ship atomic absorption spectrophotometry(GFAAS) was used to measuring blood lead level.According to the standard and regulation for blood lead detection from NCCLS and bureau of health,following performances were evaluated:inprecision,inaccuracy,method checking line,quantitative checking line,reportable range,cross contamination etc.Results: The blood lead level was highly consistent with adsorption value,the coefficient r=0.999 9(t=103.06,P<0.01);the inprecision,inaccuracy,methods test line and quantitative test line all meet the clinical need.The quantitative data could be provided when the blood lead level below 298 μg/L.Average cross contamination rates were 0.378%-0.394%.Experiment result fulfilled all demand for performance evaluation.Conclusions: Successfully establish measurement protocol with stable,repeatable and significant clinical development value.

       

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