GOLIM4、miR-153-3p在鼻咽癌组织中表达与病人临床病理特征的关系

涂恩毅; 林艳; 严欢

GOLIM4、miR-153-3p在鼻咽癌组织中表达与病人临床病理特征的关系

The relationship between the expression of GOLIM4 and miR-153-3p in nasopharyngeal carcinoma tissues and clinicopathological characteristics of patients

摘要

摘要:
目的： 探讨miR-153-3p、高尔基体整合膜蛋白4（GOLIM4）在鼻咽癌组织中的表达与病人临床病理特征的关系。
方法： 选取手术治疗的102例鼻咽癌病人为观察组，收集手术病人的癌组织，选择因淋巴组织增生进行鼻咽活检、病理检查为鼻咽慢性炎症病人的活检标本（无癌鼻咽组织）102例为对照组。实时荧光定量PCR（qRT-PCR）法检测miR-153-3p水平，采用免疫组织化学染色法检测GOLIM4分布情况；Western blotting法检测组织中GOLIM4水平；分析miR-153-3p、GOLIM4表达情况与临床病理学指标的关系；利用Kaplan-Meier法分析miR-153-3p、GOLIM4表达水平与病人预后的关系。
结果： GOLIM4在癌组织中的表达较对照组中升高，miR-153-3p表达较对照组降低，二者表达呈负相关关系（r = –0.523，P ＜ 0.05）。鼻咽癌病人癌组织中GOLIM4、miR-153-3p表达与年龄、性别、肿瘤直径、病理类型无关，与分化程度、淋巴结转移、远处转移、临床分期、T分期有关（P ＜ 0.05）。Kaplan-Meier分析结果显示，GOLIM4高表达组病人3年累计生存率显著低于低表达组（P ＜ 0.05）；miR-153-3p高表达组病人3年累计生存率显著高于低表达组（P ＜ 0.01）。
结论： 鼻咽癌病人癌组织中miR-153-3p呈低表达，GOLIM4呈高表达，二者表达与鼻咽癌病人分化程度、淋巴结转移、远处转移、临床分期、T分期及病人预后关系密切。

Abstract:
Objective To investigate the relationship between the expression of mir-153-3p and Golgi integral membrane protein 4 (GOLIM4) in nasopharyngeal carcinoma tissues and clinicopathological characteristics of patients.
Methods A total of 102 nasopharyngeal carcinoma patients treated with surgery were selected as the observation group, and the cancer tissues of surgical patients were collected. One hundred and two patients with lymphoid tissue hyperplasia were detected using the nasopharyngeal biopsy, the chronic inflammation tissues (no nasopharyngeal carcinoma tissues) were selected as the control group. The Real-time fluorescence quantitative PCR (qRT-PCR) was used to detect the miR-153-3p level, and the immunohistochemical staining was used to detect the distribution of GOLIM4. The level of GOLIM4 in tissues was detected by Western blotting. The relationship between the expression of miR-153-3p and GOLIM4 and clinicopathological indexes was analyzed. The Kaplan-Meier method was used to analyze the relationship between the expression levels of miR-153-3p and GOLIM4 and prognosis of patients.
Results The expression level of GOLIM4 in cancer tissues was higher than that in the control group, and the expression level of mir-153-3p was lower than that in the control group, and the expression of GOLIM4 was negatively correlated with mir-153-3p (r = –0.523, P ＜ 0.05). The expression of GOLIM4 and mir-153-3p in nasopharyngeal carcinoma tissues were not related to the age, gender, tumor diameter and pathological type, but related to the differentiation, lymph node metastasis, distant metastasis, clinical stage and T stage (P ＜ 0.05). The results of Kaplan Meier analysis showed that the 3-year cumulative survival rate in the high GOLIM4 expression group was significantly lower than that in low expression group (P ＜ 0.05), and the 3-year cumulative survival rate in the mir-153-3p high expression group was significantly higher than that in low expression group (P ＜ 0.01).
Conclusions The expression of mir-153-3p is low, and the GOLIM4 is high in the cancer tissues of patients with nasopharyngeal carcinoma. The expression of mir-153-3p and GOLIM4 are closely related to the differentiation degree, lymph node metastasis, distant metastasis, clinical stage, T stage and prognosis of patients with nasopharyngeal carcinoma.

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