The clinical exploration of HK6 and B7-H4 proteins in the diagnosis of varian carcinoma
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摘要: 目的:分别检测卵巢正常和卵巢良性病变和卵巢癌组织及血清中HK6、B7-H4表达水平,探讨HK6和B7-H4联合诊断卵巢癌的临床意义。方法:免疫组织化学SP法和酶联免疫吸附实验检测卵巢组织及血清中HK6和B7-H4表达水平,并结合临床病理资料分析28例卵巢癌中HK6和B7-H4在组织表达与血清浓度之间相关性。结果:HK6在卵巢癌组织中呈阳性表达,但B7-H4在黏液性癌中呈阴性表达。HK6和B7-H4在卵巢正常患者和卵巢良性病变患者血清中低表达,在卵巢癌患者血清中相对较高表达。卵巢癌患者血清中HK6和B7-H4两种蛋白的表达有明显的正相关关系(P0.01)。结论:HK6和B7-H4在卵巢癌组织及血清中均较高表达,两者联合可指导卵巢癌诊断。Abstract: Objective:To detect the protein expressions of HK6 and B7-H4 in tissue and serum of normal ovary,benign ovarian disease and ovarian cancer,and explore the clinical diagnostic significance of HK6 and B7-H4.Methods:Immunohistochemical SPmethod and ELSIA were used in detecting HK6 and B7-H4 expressions in ovarian tissue and serum,and the correlation between serum concentrations of HK6 and B7-H4 expressions in ovarian tissue were analyzed combined with clinical data on 28 ovarian cancer patients.Results:HK6 was expressed positively in ovarian caricinoma,while B7-H4 was expressed negatively in mucinous carcinomas.HK6 and B7-H4 were present at low level in serum of healthy controls and benign ovarian disease patients,but expressed at high levelin serum from ovarian cancer patients.There was significant correlation between the expressions of the two proteins in ovarian cancer(P0.01).Conclusions:HK6 and B7-H4 in ovarian cancer tissue and serum are highly expressed,it can guide for diagnosis of ovarian cancer that detection of combined with the two proteins.
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Keywords:
- ovarian neoplasm /
- HK6 /
- B7-H4
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