Abstract: Objective:To investigate the sexual health knowledge and sexual-related behavior of college students in Anhui and Sichuan.Methods:The sexual health knowledge scale and sexual-related behavior questionnaires were used.All data were analyzed by SPSS16.0.Results:The results revealed that college students in the two regions were quitely lack of sexual health knowledge,and the sexual health knowledge level of female college students was significantly lower than that of male college students.Sexual-related behavior active showed diversity trends,male students had significantly more sexual activities than women,including homosexual communication,forced sexual intercourse and so on.Conclusions:It suggested that Chinese college students need sex education urgently,especially sexual health knowledge education for female students,sexual health behavior education for male students.Besides,sexual and gender diversity,gender education,sexuality and law,and sexuality and sociality education should also be concerned.