血清C反应蛋白测定的方法学比较

Comparative study of the methods of detecting serum C reactive protein

摘要: 目的:对全自动干化学法测定血清C反应蛋白(CRP)进行应用评价。方法:在VITROS-250型干式生化分析仪上测定血清CRP,并同时在日立7600型全自动生化分析仪上检测。结果:血清CRP的检测限为7.0～110.0 mg/L;高、中、低浓度的批内CV分别为1.79%、1.90%和2.46%,批间CV分别为1.95%、2.53%和3.62%;回收率平均为98.2%;与日立7600型全自动生化分析仪相关系数R2=0.989 5,相关方程为Y=0.970 2X+0.431 9;血红蛋白2.0 g/L,胆红素浓度356mol/L,三酰甘油浓度7.8 mmol/L,对全自动干化学法测定基本无干扰。结论:干化学法测定CRP快速、准确、可靠。

Abstract: Objective:To evaluate the automatic dry chemistry method in detecting serum C reactive protein(CRP).Methods:The serum CRP was detected by the VITROS-250 dry chemistry analyzer and the HITACH-7600 automatic chemistry analyzer simultaneously.Results:The linear range of the dry chemistry method was 7.0-110.0 mg/L;the high,mean and low value within and between-run CV were 1.79%,1.90%,2.46% and 1.95%,2.53%,3.62% respectively.The average recovery rate was 98.2%;the correlation coefficient and correlation equation to the HITACH-7600 automatic chemistry analyzer were R2=0.989 5,Y=0.970 2X+0.431 9.There was no significant interference in the assay when hemolysin2.0 g/L,bilirubin356 mol/L and triglyceride7.8 mmol/L.Conclusions:Automatic dry chemistry is a fast,accurate and stable method for the detection of CRP.