猪带绦虫六钩蚴TSO45-4B抗原FnⅢ结构域相应的线性B细胞表位肽免疫原性研究

Research on immunity response about Taenia solium oncosphere TSO45-4B antigens FnⅢ structure domain linear B cells epitope peptides

摘要: 目的:观察载体蛋白偶联的TSO45-4B抗原FnⅢ结构域相应的线性B细胞表位肽诱导的体液免疫反应。方法:人工合成TSO45-4B抗原FnⅢ结构域2条预测表位肽,偶联钥孔血蓝蛋白免疫小鼠,采用ELISA法检测小鼠血清中预测表位肽特异性抗体滴度。结果:免疫小鼠血清中检测到1条预测表位肽特异性抗体,其效价达到1∶1 280。结论:设计的1条TSO45-4B抗原FnⅢ结构域线性B细胞表位肽可诱导小鼠产生体液免疫反应。

Abstract: Objective: To observe the humoral immune response induced by Taenia solium oncosphere TSO45-4B antigens FnⅢ structure domain linear B cells epitope peptides in mice.Methods: The two predicted B cell epitope peptides of TSO45-4B Fn Ⅲ structure domain conjugated with carrier protein of keyhole limpet hemocyanin were synthesized and used to immunize mice.The mice specific serum antibody titer to the epitope peptides synthesized was tested by ELISA.Results: The specific antibody to one of the predicted epitope peptides synthesized was found in mice serum,and the titer was 1∶ 1 280.Conclusions: One of the two predicted linear B cell epitope peptides of TSO45-4B FnⅢ structure domain can induce the humoral immune response in mice.