Expressions and significance of p16 and HER-2 proteins in human gastric carcinoma
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摘要: 目的:探讨p16和HER-2蛋白在胃癌组织中的表达及其与临床病理参数的关系。方法:采用免疫组织化学S-P法检测60例胃癌及其30例胃正常组织中p16和HER-2蛋白的表达,并分析其与胃癌临床病理参数之间的关系。结果:p16在胃癌组织中的阳性率为21.7%,低于胃正常组织中的73.3%(P0.01);p16在无淋巴结转移、Ⅰ～Ⅱ期胃癌患者的阳性率分别高于有高、中分化、Ⅲ～Ⅳ期患者(P0.05和P=0.05)。HER-2蛋白在胃癌组织中阳性率为38.3%,高于在胃正常组织中的0.00%(P0.01);HER-2蛋白在高、中分化、有远处转移和Ⅲ～Ⅳ期胃癌患者的阳性率分别高于低、未分化、无远处转移和Ⅰ～Ⅱ期患者(P0.05～P0.01)。p16和HER-2蛋白阳性之间无相关关系(P0.05)。结论:p16蛋白的失表达和HER-2蛋白的过表达可能与胃癌的转移进展有关,对评估胃癌生物学行为及判断预后具有重要意义。Abstract: Objective:To investigate the expressions of p16 and HER-2 proteins in human gastric carcinoma tissues and its correlation with clinical pathological factors.Methods:The expressions of p16 and HER-2 proteins in 60 cases of gastric carcinoma and 30 cases of normal gastric tissues were detected using streptavidin-peroxidase immunohistochemistry method,and the relation between the expressions and the clinicopathological parameter of gastric carcinoma was analyzed.Results:The positive rates of p16 in human gastric carcinoma and normal tissues were 21.7% and 73.3%,respectively(P0.01).The expression of p16 protein in gastric carcinoma tissues with no lymphatic metastasis and stageⅠ-Ⅱ TNM were higher than those with lymphatic metastasis(P0.05) and stage Ⅲ-Ⅳ TNM(P0.05 and P=0.05);The positive rates of HER-2 in human gastric carcinoma and normal tissues were 38.3% and 0.00%,respectively(P0.01).The expression of HER-2 protein in gastric carcinoma tissues with high,middle differentiation,remote metastasis and stage Ⅲ-Ⅳ TNM were respectively higher than those with no low,no differentiation,no remote metastasis or stage Ⅰ-Ⅱ TNM(P0.05-P0.01).The expression of p16 protein was not correlated with HER-2 protein in human gastric carcinoma tissues(P0.05).Conclusions:Loss-expression of p16 and over-expression of HER-2 may be correlated with the metastasis and development of gastric carcinogenesis.Both of them are of great significance in evaluating the biological behavior and prognosis of gastric carcinoma.
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Keywords:
- gastric neoplasms /
- p16 /
- HER-2 /
- immunohistochemistry
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