Abstract: Objective: To explore the prevalence status and molecular features of astrovirus diarrhea in infants from Jilin and Lanzhou.Methods: The astrovirus expressions in 417 diarrhea specimens from Jinlin and 290 specimens from Lanzhou were detected by one-step PCR.Partial nucleotide fragments in ORF1b region of astrovirus genome were screened,which were somatotypedbg using partial ORF2 sequences.Results: Seventeen postive astrovirus cases in 707 diarrhea specimens,which included 7 cases from Jilin and 10 cases from Lanzhou were detected,the total postive rate was 2.4%.The postive ORF2 specimens in 12 cases were HAstV 1,which belonged to Lineage 1b cluster except 1 Lineage 1a cluster.The HAstV infection mainly distributed in infants less than 2 years,the main morbidity season in Jinlin and Lanzhou were Spring and Autumn,respectively.Conclusions: Astrovirus is the main pathogen of virus diarrhea in infants,HAstV 1 is common serotype,children less than two years old are high-risk group and main morbidity season is difference in different areas.