Abstract: Objective:To observe the expressions of the special AT-rich sequence-binding protein 1(SATB1) in different hepatoma cell lines and the effects of Cisplatin on the morphology and SATB1 expression of HepG2 cells.Methods:The mRNA expressions of SATB1 of three hepatoma cell lines,HepG2,BEL-7402 and SMMC-7721 were detected using reverse transcription polymerase chain reaction.After HepG2 cells were treated with different concentrations(2.5,5.0 and 10.0 g/ml) of Cisplatin for 24 hours,the cell morphology were observed with inverted microscope and the mRNA expressions of SATB1 were examined using RT-PCR.Results:The expressions of SATB1 in BEL-7402,SMMC-7721 and HepG2 had no statistically significant difference(P0.05).The changes of cell morphology,the decreasing in cell number and increasing in cell damage and death were observed under inverted microscope after HepG2 cells were treated with Cisplatin for 24 hours.The mRNA expressions of SATB1 decreased gradually with the increasing concentrations of Cisplatin,and the mRNA expressions of SATB1 treated with 5.0 and 10.0 g/ml of Cisplatin had statistical significance compared with that of control group(P0.05).Conclusions:All three hepatoma cell lines can express SATB1.Cisplatin can inhibit the proliferation of HepG2 cells and the expression of SATB1,so as to inhibit the growth of hepatoma cells.