凌云志, 禹莉, 梁启胜, 李晓红, 王南海. 七氟醚对兔肝脏缺血再灌注损伤的影响[J]. 蚌埠医学院学报, 2012, 36(8): 884-887.
    引用本文: 凌云志, 禹莉, 梁启胜, 李晓红, 王南海. 七氟醚对兔肝脏缺血再灌注损伤的影响[J]. 蚌埠医学院学报, 2012, 36(8): 884-887.
    LING Yun-zhi, YU Li, LIANG Qi-sheng, LI Xiao-hong, WANG Nan-hai. Protective effects of sevoflurane on liver ischemia reperfusion injury in rabbits[J]. Journal of Bengbu Medical College, 2012, 36(8): 884-887.
    Citation: LING Yun-zhi, YU Li, LIANG Qi-sheng, LI Xiao-hong, WANG Nan-hai. Protective effects of sevoflurane on liver ischemia reperfusion injury in rabbits[J]. Journal of Bengbu Medical College, 2012, 36(8): 884-887.

    七氟醚对兔肝脏缺血再灌注损伤的影响

    Protective effects of sevoflurane on liver ischemia reperfusion injury in rabbits

    • 摘要: 目的:探讨七氟醚对兔肝脏缺血再灌注损伤的影响。方法:将21只新西兰大白兔随机分为假手术组(S组)、肝脏缺血再灌注组(IR组)和七氟醚缺血再灌注组(Sev-IR组)。S组仅行肝脏游离不阻断肝血流;IR组在夹闭肝门血管缺血45 min后再灌注60 min;Sev-IR组在七氟醚麻醉后,夹闭肝门血管缺血45 min后再灌注60 min。分别检测3组兔肝组织中丙二醛(MDA)、黄嘌呤氧化酶(XOD)的含量;HE染色观察肝组织形态学改变;免疫组织化学染色检测c-fos蛋白的表达情况。结果:肝缺血再灌注后,MDA、XOD含量均显著升高;光镜下见肝窦明显扩张、淤血,肝细胞明显水肿变性和空泡状变性,并有点状坏死;c-fos蛋白大量阳性表达。与IR组比较,Sev-IR组MDA、XOD含量均下降,差异均有统计学意义(P0.05);肝细胞变性、坏死及肝血窦淤血减轻;肝组织c-fos蛋白表达量明显下调(P0.05)。结论:肝缺血再灌注损伤可使大量氧自由基释放,c-fos蛋白表达增加,引起肝组织的损伤;七氟醚可减轻肝缺血再灌注后肝组织损伤程度,可能与抑制再灌注后的过度氧化反应和减少c-fos蛋白表达有关。

       

      Abstract: Objective:To investigate the effects of sevoflurane on liver ischemia reperfusion injury in rabbits.Methods:Twenty-0ne rabbits were randomly divided into sham operation group(S),ischemia reperfusion group(IR) and sevoflurane ischemia reperfusion group(Sev-IR).Rabbits in IR group and Sev-IR group suffered from ischemia for 45 minutes and were followed by reperfusion for 60 minutes.Sev-IR group was anesthetized with sevoflurane.The concentrations of MDA and XOD of liver tissue were determined.The histopathologic changes in the liver were also observed.The expressions of c-fos protein were observed through immunohistochemisty.Results:The concentrations of MDA and XOD increased significantly after liver ischemia-reperfusion.The sinusoid was expanded and congestion obviously.The hepatic cells were hydropic degeneration and vacuole degenerated.Some of them were spotty necrosis.The protein of c-fos was positive expression.When using sevoflurane,the concentrations of MDA and XOD were statistical significantly decreased compared with IR group(P0.05).The hepatic histopathological changes were improved.The degree of degeneration and necrosis of hepatic cells and the congestion of hepatic sinusoid lessened.The expression of c-fos was down regulated significantly(P0.05).Conclusions:The injury of liver ischemia reperfusion can release mass oxygen radicals and increase the expression of c-fos.This can induce hepatic tissue injury.Sevoflurane can protect liver from ischemia reperfusion injury.Its possible mechanisms are restraining excess oxidizing reaction and decreasing the expression of c-fos protein.

       

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