管英华, 魏晓巍, 谢扬虎, 李见, 王露, 李玉云, 张强. 人脐带间充质干细胞对再生障碍性贫血小鼠造血的影响[J]. 蚌埠医学院学报, 2013, 37(6): 648-651.
    引用本文: 管英华, 魏晓巍, 谢扬虎, 李见, 王露, 李玉云, 张强. 人脐带间充质干细胞对再生障碍性贫血小鼠造血的影响[J]. 蚌埠医学院学报, 2013, 37(6): 648-651.
    GUAN Ying-hua, WEI Xiao-wei, XIE Yang-hu, LI Jian, WANG Lu, LI Yu-yun, ZHANG Qiang. Effect of mesenchymal stem cells derived from human umbilical cord on hemopoiesis of mice with aplastic anemia[J]. Journal of Bengbu Medical College, 2013, 37(6): 648-651.
    Citation: GUAN Ying-hua, WEI Xiao-wei, XIE Yang-hu, LI Jian, WANG Lu, LI Yu-yun, ZHANG Qiang. Effect of mesenchymal stem cells derived from human umbilical cord on hemopoiesis of mice with aplastic anemia[J]. Journal of Bengbu Medical College, 2013, 37(6): 648-651.

    人脐带间充质干细胞对再生障碍性贫血小鼠造血的影响

    Effect of mesenchymal stem cells derived from human umbilical cord on hemopoiesis of mice with aplastic anemia

    • 摘要: 目的:通过从脐带中分离和培养脐带间充质干细胞(MSCs),探讨其对再生障碍性贫血(再障)小鼠骨髓造血的影响。方法:原代贴壁法培养人脐带MSCs,将小鼠分为模型组、对照组、MSCs输注组进行实验。经静脉输注免疫介导方法建立再障模型小鼠,分别于3、7、14、21、28d观察其外周血象指标变化和骨髓组织形态;ELISA检测造血负调控因子(IFN-)的水平,计数骨髓造血干细胞集落生成单位。结果:原代培养中脐带组织块直接贴壁后8~10d,边缘爬出长梭形MSCs,随传代培养呈平行、漩涡状生长;再障小鼠经MSCs输注治疗后外周血象明显增高,IFN-水平下降(P0.01);治疗组骨髓红细胞集落形成单位与粒-巨噬细胞集落形成单位均较模型组明显增高(P0.01),成纤维细胞集落形成单位计数差异无统计学意义(P0.05)。结论:人脐带来源的MSCs对再障模型小鼠骨髓可发挥造血支持作用。

       

      Abstract: Objective:To investigate the effects of umbilical cord mesenchymal stem cells(MSCs)on hemopoietic function of bone marrow in mice with aplastic anemia(AA).Methods:Umbilical cord MSCs were primarily cultured with adherent method.All mice were divided into the model group,control group and MSCs infusion group.The aplastic anemia model mice established by immune-mediated means were infused with MSCs through caudalis vena.The peripheral hemogram and bone marrow morphology were observed at 3,7,14,21 and 28 days.The level of hematopoiesis negative regulatory factor(IFN-)was detected by ELISA.The colony-forming unit counts of bone marrow hemopoietic progenitor cells were measured.Results:The spindle-shaped MSCs extended from the tissue edge at 8-10 days after primary adherent culture of umbilical cord,and those were parallelly or whirlpool-shape growth with the cultivation.The increasing peripheral hemogram and decreasing IFN- levels of AA mice treated with MSCs were significant(P0.01).The colony forming units-erythroid and colony forming units-granulocyte macrophage of the treatment group were significantly higher than those in the control group(P0.01),but there was no significant difference in the colony forming units-fibroblast count between the two groups(P0.05).Conclusions:MSCs derived from human umbilical cord can play some poles on hemopoietic function of the bone marrow of AA mice.

       

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