Abstract: Objective: To investigate the correlation of the expression of 67 kDa laminin receptor(67LR) with its biological behavior of gallbladder carcinoma.Methods: The 67LR protein expression in 40 cases of gallbladder carcinoma,21 cases of chronic cholecystitis and 9 cases of gallbladder adenoma were detected by immunohistochemistry SP method.Results: The positive expression rate of 67LR was 52.50% in the cases with gallbladder carcinoma,which was significantly higher than that with chronic cholecystitis(P<0.01),whereas no significant difference was detected between the cases with gallbladder adenoma or carcinoma(P>0.05).The positive expression rate of 67LR in gallbladder carcinoma with lymph node metastasis was significantly higher than that without metastasis((P<0.005)).Conclusions: 67LR may play an important role in both premalignant transformation of gallbladder adenoma and acquisition of the invasive and metastasis ability.