Abstract: Objective:To investigate the distribution and antibiotic resistance of pathogens that cause pulmonary infections among patients in the intensive care unit(ICU),and provide basis for rational selection of clinical drugs.Methods:The clinical date of 1 425 bacterial strains isolated from the sputum of patients with pulmonary infection in the ICU from Aug.2008 to Feb.2012 were retrospectively analyzed and validated exclusively with the CLSI standard.Results:Of the infection pathogens,gram-negative bacteria accounted for 64.8%,gram-positive cocci 19.6% and fungi 15.6%.The common clinical isolates in ICU were Acinetobacter baumannii,Staphylococcus aureus,Pseudomonas aeruginosa,Candida albicans,Klebsiella pneumoniae,Escherichia coli,Staphylococcus epidermidis and Candida glabrata,all of which showed high resistance to the antibiotics except fungi.Conclusions:The pathogen in ICU is diverse.Multidrug resistant strains are the focus of concern.