Abstract: Objective:To monitor the distribution and drug resistance of clinical isolated common non-fermentative Gram-negative bacilli,so as to provide basis for appropriate use of antibiotics in clinic.Methods:The 728 strains common non-fermentative gram-negative bacilli were collected and their antimicrobial susceptibility was studied.The identification and drug susceptibility tests were detected by paper-disc agar-diffusion method and minimal inhibitory concentration method.The results were evaluated in accordance with the standard of American Clinical Laboratory Information Systems.Results:In the 728 strains non fermentative Gram-negative bacilli,the dominant four strains were Acinetobacter baumannii ( 330 strains ) firstly,Pseudomonas aeruginaosa ( 290 strains ),Stenotrophomonas maltophilia ( 38 strains) and Burkholderia cepacia ( also 38 strains).The main bacterias were most from sputum system,totally 543 strains and the most distribution were detected in intensive care unit ( 308 strains ).The resistant rates of Pseudomonas aeruginosa to imipenem cefoperaxone/sulbactam,piperacillin/tazobactam were all less than 30%.The resistant rates of Acinetobacter baumannii were 31.2% to minocycline,53.94% to cefoperaxone/sulbactam and all lower than others.The resistant rate of Stenotrophomonas maltophilia to minocycline was 5.3%.The resistant rates of Burkholderia cepacia to cotrimoxazole and minocycline were lower than others,21.05% and 15.8% respectively.Conclusions:Different kinds of nonfermentative Gram negative bacilli had a greater difference in drug sensitivity.