Expression of WT1 gene in bone marrow of acute leukemia patients
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摘要: 目的：探讨急性白血病(AL)患者骨髓细胞WT1基因的表达。方法：采用实时荧光定量PCR(RQ-PCR)检测100例AL患者骨髓细胞WT1基因及内参基因(ABL)，以WT1基因拷贝数/内参基因拷贝数(NQ比值)计算基因表达水平。结果：初诊组和复发组AL患者WT1表达水平明显高于完全缓解组(P0.01)。初诊患者中，AL各亚型之间WT1表达水平差异均有统计学意义(P0.01)，急性淋巴细胞白血病WT1表达水平最低，急性早幼粒细胞白血病表达最高。随访3例患者，达到完全缓解时WT1表达很低，复发时WT1表达均再次升高。结论：WT1基因在AL中的表达，可作为白血病疗效评价及监测微小残留病灶的指标。
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关键词:
- 白血病 /
- WT1基因 /
- 微小残留病 /
- 实时荧光定量聚合酶链反应
Abstract: Objective: To investigate the expression level of Wilms'tumor 1(WT1) gene in bone marrow of acute leukemia(AL) patients.Methods:The copy numbers of WT1 and reference gene in BM of 100 patients with AL were measured by real-time quantitative polymerase chain reaction (RQ-PCR).The gene level was determined by the ratio of the copy numbers of WT1 and reference gene.Results:The expression levels of WT1 in the newly diagnosed and relapsed AL patients were significantly higher than that in the complete remission(CR) AL patients(P0.01).In newly diagnosed AL patients,the WT1 expression difference in different subtype of AL was statistical significance(P0.01),the WT1 expression was the lowest in acute lymphocytic leukemia and the highest in acute promyelocytic leukemia.Three patients were followed up,the WT1 gene expression was low in the remission period,and increased after relapse.Conclusions:WT1 expression level in AL patients can be a marker for detecting minimal residual disease and evaluating therapy efficacy. -
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