T17和T22细胞在健康成人外周血中的比例
The proportions of T17 cells and T22 cells in healthy adult peripheral blood
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摘要: 目的:观察健康成人外周血中T细胞和T细胞中辅助性T细胞17(TH17)样细胞和TH22样细胞的比例。方法:采集18名正常人外周血用荧光抗体染色后用流式细胞仪检测T细胞、T细胞中白细胞介素-22(IL-22)、IL-17和干扰素(IFN-)表达细胞的百分比。结果:将正常人外周新鲜全血中CD3+T细胞分成T细胞和T细胞两群。TH1样细胞在T细胞中比例为14.31%,明显低于在T细胞中的61.07%(P0.01),而TH17样细胞在T细胞中的比例为1.16%,明显高于在T细胞中的0.27%(P0.01),TH22样细胞在T细胞中与T细胞中的比例分别为1.06%和0.91%,差异无统计学意义(P0.05)。结论:在T细胞中存在IL-17和IL-22均阳性的T17细胞和仅产生IL-22的T22细胞,但比T细胞中的TH17样细胞和TH22样细胞少。Abstract: Objective: To observe the proportions of TH17-like cells and TH22-like cells in T cells and T cells in healthy adult peripheral blood.Methods: Eighteen healthy adult peripheral blood samples were stained with fluorescent antibody and the percentages of interleukin-22， interleukin-17 and IFN--producing cells in T cells and T cells were detected by flow cytometry.Results: The CD3 + T cells in the whole blood cells were divided into two groups: T cells and T cells.The proportions of TH1-like cells，TH17- like cells and TH22-like cells in T cells were 14.31%，1 .16% and 1.06%， respectively.While the proportions of TH1-like cells， TH17-like cells and TH22-like cells in T cells were 61.07%，0.27% and 0.91%， respectively.The proportion of TH1-like cells in T cells was lower than in T cells， and the proportion of TH17-like cells in T cells was higher than in T cells (P0.01) .There were no statistical differences about the proportion of TH22-like cells in T cells and in T cells(P0.05) .Conclusions: There exists IL-17 and IL-22 double-positive T17 cell subpopulation and IL-22 single-positive T22 cell subpopulation in T cells.But the proportions are lower than those in T cells.
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