周艳梅, 江从军, 张汝芝, 柴继侠, 贺文欣, 陈晓宇, 陈晓蓉. NF-B特异性抑制剂PDTC对鼠黑素瘤B16细胞增殖及VEGF表达的影响[J]. 蚌埠医学院学报, 2012, 36(9): 1013-1015.
    引用本文: 周艳梅, 江从军, 张汝芝, 柴继侠, 贺文欣, 陈晓宇, 陈晓蓉. NF-B特异性抑制剂PDTC对鼠黑素瘤B16细胞增殖及VEGF表达的影响[J]. 蚌埠医学院学报, 2012, 36(9): 1013-1015.
    ZHOU Yan-mei, JIANG Cong-jun, ZHANG Ru-zhi, CHAI Ji-xia, HE Wen-xin, CHEN Xiao-yu, CHEN Xiao-rong. The protective effect of diazoxide preconditioning on myocardial ischemia-reperfusion injury and the influence on connexin 43[J]. Journal of Bengbu Medical College, 2012, 36(9): 1013-1015.
    Citation: ZHOU Yan-mei, JIANG Cong-jun, ZHANG Ru-zhi, CHAI Ji-xia, HE Wen-xin, CHEN Xiao-yu, CHEN Xiao-rong. The protective effect of diazoxide preconditioning on myocardial ischemia-reperfusion injury and the influence on connexin 43[J]. Journal of Bengbu Medical College, 2012, 36(9): 1013-1015.

    NF-B特异性抑制剂PDTC对鼠黑素瘤B16细胞增殖及VEGF表达的影响

    The protective effect of diazoxide preconditioning on myocardial ischemia-reperfusion injury and the influence on connexin 43

    • 摘要: 目的:探讨核因子-B(NF-B)特异性抑制剂吡咯烷二硫代氨基甲酸盐(PDTC)对鼠黑素瘤B16细胞增殖及血管内皮生长因子(VEGF)表达的影响。方法:不同浓度的PDTC作用于B16细胞不同时间后,MTT法测定其对B16细胞增殖的抑制率,ELISA检测PDTC作用下B16细胞VEGF的表达情况。结果:经PDTC处理的实验组,肿瘤细胞增殖活性明显受到抑制(P0.01);与对照组相比,PDTC作用后B16细胞VEGF表达降低(P0.01)。结论:PDTC具有显著抑制鼠黑素瘤B16细胞生长及VEGF表达的作用,是一种有潜力的抑制黑素瘤增殖的药物。

       

      Abstract: Objective: To observe the protective effect of diazoxide preconditioning on rat myocardial ischemia-reperfusion injury in vivo and observe the effect of diazoxide preconditioning on connexin 43 (Cx43) .Methods: Forty-five male SD rats were randomly divided into five groups: sham group, IRI group, IPC group,DPC group and 5-HD group,9 rats in each group.Myocardial ischemiareperfusion injury model was established by ligation of left anterior descending coronary artery for 30 minutes, followed by 120 minutes of reperfusion.The left ventricular systolic pressure, left ventricular diastolic pressure and other heart function were measured by Medlab biological signal collecting and processing system.After reperfusion,myocardial enzymes in plasma were tested; and then the expressions of TCx43 and PCx43 of ischemia-reperfusion myocardium were studied by western blot.Results: Compared with IRI group, the levels of CK-MB and LDH were decreased with significant statistical difference in DPC group(P0.05-P0.01) ; the expression level of Cx43 protein was significantly higher, especially of PCx43(P0.01) ; and left ventricular systolic and diastolic function were increased (P0.05-P0.01) .Conclusions: Diazoxide preconditioning can effectively retain the expression level of Cx43 of left ventricular myocardium caused by ischemia-reperfusion, reduce myocardial injury, and maintain systolic and diastolic function of left ventricle.Cx43 may be involved in the protective effect of diazoxide preconditioning.

       

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