李恒, 高霞. 转化生长因子-1及结缔组织生长因子在病毒性心肌炎中的表达[J]. 蚌埠医学院学报, 2012, 36(9): 1020-1023.
    引用本文: 李恒, 高霞. 转化生长因子-1及结缔组织生长因子在病毒性心肌炎中的表达[J]. 蚌埠医学院学报, 2012, 36(9): 1020-1023.
    LI Heng, GAO Xia. Expressions of transforming growth factor-1 and connective tissue growth factor in viral myocarditis[J]. Journal of Bengbu Medical College, 2012, 36(9): 1020-1023.
    Citation: LI Heng, GAO Xia. Expressions of transforming growth factor-1 and connective tissue growth factor in viral myocarditis[J]. Journal of Bengbu Medical College, 2012, 36(9): 1020-1023.

    转化生长因子-1及结缔组织生长因子在病毒性心肌炎中的表达

    Expressions of transforming growth factor-1 and connective tissue growth factor in viral myocarditis

    • 摘要: 目的:探讨转化生长因子-1(TGF-1)及结缔组织生长因子(CTGF)在病毒性心肌炎(VM)中的表达。方法:雄性Balb/c小鼠36只随机均分为对照组和模型组。模型组建立VM模型。分别于7、14、28 d每组随机抽取6只小鼠断颈处死。心脏称重后用4%多聚甲醛固定,苏木精-伊红染色,光镜下观察心肌组织形态学改变,半定量计算病理积分。免疫组织化学方法检测心肌Ⅰ型和Ⅲ型胶原的蛋白表达,并应用病理图像分析仪进行图像分析,分别计算Ⅰ型和Ⅲ型胶原的面积。心肌组织CTGF、TGF-1同样采用免疫组织化学测定,免疫组织化学图像分析经标准灰度校正后测定阳性细胞的吸光度及面积,以两者乘积表示组织中该抗原相对含量。结果:与对照组比较,模型组病理积分、心肌Ⅰ型和Ⅲ型胶原蛋白表达、心肌组织中CTGF、TGF-1的表达均显著增加(P0.01)。心肌Ⅲ型胶原蛋白的表达与CTGF、TGF-1呈正相关关系(r=0.612,0.533,P0.05)。TGF-1与CTGF表达也呈正相关关系(r=0.939 P0.05)。结论:TGF-1及CTGF协同参与了VM心肌纤维化的发生、发展过程。

       

      Abstract: Objective: To investigate the expressions of transforming growth factor-1(TGF-1) and connective tissue growth factor (CTGF) in viral myocarditis.Methods: Thirty-six male Balb /c mice were divided into two groups randomly: control group and model group.Model group was established by Cox-sackie virus B3 induced viral myocarditis.Six mice in each group were killed on days 7, 14 and 28 respectively.The hearts were weighed and fixed with 4% paraform,histological cross sections were stained with hematoxylineosin and myocardial histopathoplogic scores were counted under optical microscope.The protein expressions of myocardial collagen Ⅰ and Ⅲ were examined by immunohistochemical method and analyzed using a computer assisted procedure, then the area of collagens were calculated.The protein expressions of TGF-1 and CTGF were also examined by immunohistochemical method, and the absorbance and area of positive cells were measured after standard gray correction, the antigen contents were calculated by product of absorbance and area.Results: In contrast to control group, the histopathoplogic scores, expressions of myocardial collagen Ⅰ and Ⅲ,TGF-1 and CTGF were increased markedly in Cox-sackie virus B3 infected group (P0.01) .The expression of myocardial collagen Ⅲ was correlated with TGF-1 and CTGF positively (P0.05) , the expression of TGF-1 was correlated with CTGF positively (P0.05) .Conclusions: TGF-1 and CTGF participate in the occurreuce and development of myocardial fibrosis of viral myocarditis.

       

    /

    返回文章
    返回