一种自制的小鼠断尾采血简易装置
A simple device for blood collection by tail snipping in mice
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摘要: 目的:探讨自制简易新装置对小鼠断尾取血的采血效果,建立简易、安全、方便、省时的小鼠采血方法。方法:用废弃的50 ml离心管、纸盒、纱布等制作2个采血装置,并列固定于同一6孔板上,对50只6~8周龄的昆明系小鼠进行重复4次断尾采血。结果:自制的固定装置价格低廉、制作简单、操作灵活,并可同时为2只小鼠采血,采血量可达0. 25~0. 50 ml,实验中小鼠无伤残、无死亡。结论:自制的小鼠固定装置采血量高、安全性好,是理想的小鼠断尾采血装置。Abstract: Objective: To investigate the effects of a new simple device for blood collection by tail snipping in mice for providing a safey, convenient and economical method. Methods: The device was made by some hard paper,gauze,and two centrifuge tubes( 50 ml) , and which were fixed on a plastic plate. The blood of 50 Kunming mice aged 6 to 8 weeks were collected by tail snipping for repeat 4 times. Results: The device was cheap, and easy to be made and handled,which could collect blood for two mice at the sametime. No mouse was disabled or dead during the experimental course. Conclusions: The device is a safe and ideal equipment for bloodcollection by tail snipping in mice.