Abstract: Objective: To investigate the virus infected patients with acute respiratory infection in Fengtai district of Beijing. Methods: The adenoviruses，human coronary virus and metapneumovirus were detected using ＲT-PCＲ. The respiratory syncytial virus， parainfluenza virus( PIV) ， rhinovirus， enterovirus and human bocavirus were detected using nested-PCＲ. The influenza A virus( IVA，including A1，A3 and swine flu type) and influenza B virus were detected using Ｒealtime-PCＲ. Results: Among 227 specimens，111specimens with postive virus were detected， the postive detection rate was 48. 90%. The influenza virus detection rate was 61. 26% inpostive virus specimens，which was the highest， IVB was given priority to， followed by influenza A3. The PIV detection rate was the second，PIV1 was given priority to， followed by PIV4， all virus infection were clustering. The positive rate in winter was higher than other seasons， the positive rate in patients with upper respiratory infection was higher than other patients. Conclusions: IVB is the main pathogeny of respiratory infection in 2011 and 2012 winter. Winter is high season of respiratory virus， the main route of virus infection is upper respiratory infection.