CDKN2A基因失活对小细胞肺癌的影响及其临床意义

    The influence and clinical significance of cyclindependent kinase inhibitor 2A gene inactivation on small cell lung cancer

    • 摘要: 目的:探讨细胞周期依赖性激酶抑制基因(CDKN2A)失活对小细胞肺癌(SCLC)的影响及其临床意义。方法:应用RT-PCR、免疫组织化学法、Western blot检测SCLC手术切除组织72例和非肿瘤肺部组织68例中CDKN2A基因及其蛋白。结果:SCLC组织CDKN2A第一、第二外显子表达明显少于非肿瘤肺组织,差异有统计学意义(P0.01);SCLC组织中CDKN2A蛋白低表达率(68.1%)明显高于非肿瘤肺组织(36.8%)(P0.01);SCLC组织CDKN2A蛋白的表达水平显著低于非肿瘤肺组织(P0.01);CDKN2A在不同病理分期以及有无淋巴结和远处转移的SCLC组织中表达差异均无统计学意义(P0.05)。结论:CDKN2A基因的变异及其编码蛋白质的失活与SCLC发生、发展有关。

       

      Abstract: Objective: To explore the influence and clinical significance of cyclindependent kinase inhibitor 2A (CDKN2A) inactivation on small cell lung cancer(SCLC) . Methods: The gene and protein expression of CDKN2A in SCLC tissue of 72 cases and non-tumor lung tissue of 68 cases were detected by the semi-quantitative RT-PCR, IHC and Western blot. Results: The CDKN2A and its first and second exon mRNA expression of SCLC were significantly less than that of non-tumor lung tissue,and there was statistical significance between their diffenence(P < 0. 01) . The protein expression rate of CDKN2A in SCLC(68. 1%) was significantly higher than that of non-tumor lung tissue(36. 8%) (P < 0. 01) . The protein expression of CDKN2A in SCLC was significantly lower than that of non-tumor lung tissue(P < 0. 05) . There was not statistical significance between the CDKN2A expression in different pathological stages and lymph node metastasis of SCLC tissue (P > 0. 05) . Conclusions: The CDKN2A gene mutation and its encoded protein inactivation are related to the development and progression of SCLC.

       

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