Drug containing serum and infected macrophages model by Mycobacterium tuberculosis for evaluation of activity of antitubercular agents
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摘要: 目的: 建立含药血清作用结核杆菌感染巨噬细胞模型的抗结核杆菌药物活性检测方法,探讨该方法在评价抗结核杆菌药物活性中的应用。方法: 以结核杆菌感染小鼠腹腔巨噬细胞,加入低剂量或高剂量异烟肼给药小鼠的含药血清共同培养数天后,破碎巨噬细胞释放可能仍存活的细菌,体外培养后检查形成的菌落数,评价异烟肼的抗结核活性,并与传统最低抑菌浓度试验进行比较。结果: 培养数天后,低剂量和高剂量异烟肼含药血清组菌落生成数均低于空白对照组(P<0.05~P<0.01)。结论: 应用含药血清及感染巨噬细胞模型可对抗结核药物活性作出较客观的评价结果。Abstract: Objective: To find a method of evaluating anti-Mycobacterium tuberculosis(TB)medicine using medicine containing serum and infected macrophage model and investigate its practicability and merits.Methods: By using the culture system constituting with serum containing isoniazid and infected macrophages,the numbers of the survival TB were confirmed through the clones of germiculture after these cells were cultured with serum containing isoniazid for several days,then the isoniazid's activity in this culture system was reflected and compared with the MIC.Results: The survival TB clones in the groups of low dose and high dose of isoniazid containing serum were remarkably fewer than those in the control(P<0.05-P<0.01).Conclusions: The medicine's function of anti-Mycobacterium tuberculosis can be objectively evaluated by using medicine containing serum and infected macrophage model.
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Keywords:
- antitubercular agents /
- models,biological /
- isoniazid /
- tubercle bacillus /
- containing medicine serum /
- macrophage
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