Abstract: Objective: To find a method of evaluating anti-Mycobacterium tuberculosis(TB)medicine using medicine containing serum and infected macrophage model and investigate its practicability and merits.Methods: By using the culture system constituting with serum containing isoniazid and infected macrophages,the numbers of the survival TB were confirmed through the clones of germiculture after these cells were cultured with serum containing isoniazid for several days,then the isoniazid's activity in this culture system was reflected and compared with the MIC.Results: The survival TB clones in the groups of low dose and high dose of isoniazid containing serum were remarkably fewer than those in the control(P<0.05-P<0.01).Conclusions: The medicine's function of anti-Mycobacterium tuberculosis can be objectively evaluated by using medicine containing serum and infected macrophage model.