Abstract: ObjectiveTo compare the effects of 10d sequential and standard triple therapy in the eradication of Helicobacter pylori ( Hp) . Methods: Ninty-two cases with chronic gastritis or peptic ulcer and positive Hp diagnosed by endoscopy were randomly divided into treatment group and control group.The control group were standard triple therapy( including 20 mg of esomeprazole，1 000 mg of amoxicillin and 500 mg of clarithromycin twice a day for 10 days.The treatment group were treated with sequential therapy( including 20 mg of esomeprazole，1 000 mg of amoxicillin for 5 days， followed by 20 mg of esomeprazole，500 mg of clarithromycin and 500 mg of tinidazole) for 5 days.Results: The eradication rates of Hp in treatment group and control group were 92.00% and 76.31% by following up， respectively， the difference of which was statistical significance( P 0.01) .The remission rate and incidence of adverse reactions between two groups were not statistical significance( P 0.05) . Conclusions: Sequential therapy can improve the eradication rate of Hp，which is economy， security and an alternative first-line treatment.