Abstract: Objective: To investigate the expressions of Twist protein (Twist) ，signal transducer and activator of transcription 3 (STAT3) ，epithelial mesenchymal transition related factor (E-cad) in gastric cancer tissue and peritumoral tissue，analyze their correlations and explore their significance in judging the prognosis of patients with gastric cancer. Methods: The expressions of Twist, STAT3 and E-cad in gastric cancer tissue and peritumoral tissue were detected by immunohistochemistry streptavidin-biotin complex (SABC) , their relationships with age, gender，histological type of tumor，metastasis and TNM stage of patient were analysed. results: The positive rate of Twist，STAT3 and E-cad in gastric cancer tissue were 57. 3%, 64. 7% and 12. 0%, respectively. The positive rate of Twist，STAT3 and E-cad in peritumoral tissue were 17. 3%, 10. 8% and 68. 0%, the expression differences of which were not statistical significance(P ＜ 0. 01) . The differences of Twist expression in different tumor metastasis, lymph node metastasis，TNM staging and depth of invasion were statistical significance(P ＜ 0. 05-P ＜ 0. 01) . There was statistically significant in the expression of STAT3 between node metastasis，depth of invasion and TNM stage(P ＜ 0. 05-P ＜ 0. 01) . They were negative correlation in Twist and STAT3 expressions and E-cad expression(P ＜ 0. 01) . Conclusions: The expressions of Twist and SATA in gastric cancer tissue have a certain relationship with tumor biological characteristics, the expression of SATA3 may activate the expression of Twist and E-cad. The detection of Twist，STAT3 and E-cad can predict the tumor invasion，metastasis and prognosis of patient.