As<sub>2</sub>O<sub>3</sub>对乳腺癌细胞的生长及端粒酶活性的影响

胡博; 陈正徐; 夏俊; 王东萍; 高绪峰

As₂O₃对乳腺癌细胞的生长及端粒酶活性的影响

1. 蚌埠医学院临床检验诊断学实验中心, 安徽蚌埠 233030;
2. 蚌埠医学院生物化学与分子生物学教研室, 安徽蚌埠 233030

基金项目:

安徽省教育厅自然科学研究资助项目(2003kj256)

详细信息

作者简介:
胡博(1982-),女,硕士研究生,研究方向:肿瘤分子生物学.

中图分类号: R737.9;O612.5
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出版历程
- 收稿日期: 2006-12-18

Influence of arsenic trioxide on breast cancer cell and telomerase activity

1. Research Center of Ninical Laboratory Science, Bengbu Medical College, Bengbu Anhui 233030, China;
2. Department of Biochemistry and Molecular Biology, Bengbu Medical College, Bengbu Anhui 233030, China

摘要

摘要: 目的:探讨不同浓度As₂O₃对人乳腺癌细胞株MDA-MB-231端粒酶及其亚单位hTERT-mRNA活性表达的影响,为临床治疗乳腺癌提供理论和实验依据。方法:将不同浓度的As₂O₃与MDA-MB-231细胞共培养不同时间后,MTT比色法检测As₂O₃对MDA-MB-231细胞的生长抑制作用;TRAP-PAGE-银染法检测As₂O₃作用后细胞端粒酶活性的改变;RT-PCR法测定MDA-MB-231细胞hTERT-mRNA的表达。结果:As₂O₃可显著抑制MDA-MB-231细胞生长,且存在浓度、时间效应关系,其24 h IC₅₀为24.73μmol/L,48 h IC₅₀为6.99μmol/L;用终浓度分别为10、20、40μmol/L的As₂O₃与MDA-MB-231细胞作用24h、48 h,银染条带显示出端粒酶活性显著下降;RT-PCR产物电泳条带显示出hTERT-mRNA的表达明显下降。结论:As₂O₃在体外可显著抑制MDA-MB-231细胞的生长,具有较明显的细胞毒作用;As₂O₃能显著抑制MDA-MB-231细胞端粒酶及其亚单位端粒酶逆转录酶的活性,且抑制作用具有明显的时间和浓度依赖关系。
- 乳腺肿瘤 /
- 三氧化二砷 /
- MDA-MB-231细胞 /
- 端粒酶 /
- 比色法
Abstract: Objective: To investigate the inhibitary effect of different concentrations of arsenic trioxide(As₂O₃) on breast cancer cells MDA-MB-231 and on the activity of telomerase hTERT-mRNA so as to provide theoretical and experimental basis for the treatment of breast cancer.Methods: MDA-MB-231 cells were cultured with As₂O₃ diflerint cokcenfrations.The growth inhibition was analyzed by MTT assay;the telomerase activity was determined by TRAP-PAGE-SILVER staining;the hTERT-mRNA expression was examined by RT-PCR assay.Results: As₂O₃ could obviously inhibit the growth of MDA-MB-231 cells,which was related with the concentrations of As₂O₃ and time of culture.Its 24-hour inhibitory concentration(IC₅₀) was 24.73 μmol/L and 48-hour inhibitory concentration(IC₅₀) was 6.99 μmol/L.Treated with As₂O₃(10-40 μmol/L) for 24-48 h,the telomerase activity and hTERT-mRNA expression of MDA-MB-231 cells were greatly inhibited.Conclusions: As₂O₃ can inhibit the growth of MDA-MB-231 cells significantly.Treated with As₂O₃,the activity of telomerase and telomerase hTERT-mRNA can be inhibited remarkably,and the effects are dependent on the concentrations and time of culture.
- breast neoplasms /
- As₂O₃ /
- MDA-MB-231 cells /
- telomerase /
- colorimetry