Abstract: Objective: To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa and extended-spectrum -lactamase (ESBLs) producing condition, gene distribution and epidemiological characteristics in clinic. Methods: The antimicrobial susceptibility was detected with the Vitek-2 compact automatic bacteria identification system, 16 antibiotics for 208 Pseudomonas aeruginosa isolated. The imipenem-resistant Pseudomonas aeruginosa were selected. ESBLs genes produced by imipenem resistance were detected by PCr, the ESBLs genes contain TEM，SHV，OXA-10，OXA-2，PEr and VEB. results: In the 208 isolates，53 strains of Pseudomonas aeruginosa were resistant to imipenem, the drug resistance rate was 25. 48%. The isolates had a high rate of drug-resistance against CTE，CZ，AMS and FTN; The PCr results showed that in the 53 imipenem-resistant Pseudomonas aeruginosa，ESBLs gene were produced in 23 strains, 16 strains were TEM，4 for OXA-10 and 3 for PEr gene; OXA-2，SHV was not detected. Conclusions: TEM of Extended-spectrum-lactamase producing in imipenem-resistant Pseudomonas aeruginosa is the main gene in our hospital，most of them are multi-drug-resistance，detecting the antimicrobial susceptibility and using antibiotics rationally is essential in clinic.