张伦军, 丁晓琳, 郭普, 钟政荣, 沈继龙. 耐亚胺培南铜绿假单胞菌的耐药性及超广谱-内酰胺酶的基因分析[J]. 蚌埠医学院学报, 2013, 37(10): 1328-1330.
    引用本文: 张伦军, 丁晓琳, 郭普, 钟政荣, 沈继龙. 耐亚胺培南铜绿假单胞菌的耐药性及超广谱-内酰胺酶的基因分析[J]. 蚌埠医学院学报, 2013, 37(10): 1328-1330.
    ZHANG Lun-jun, DING Xiao-lin, GUO Pu, ZHONG Zheng-rong, SHEN Ji-long. The antibiotic-resistance and its gene analysis of extended-spectrum -lactamases in imipenem-resistant Pseudomonas aeruginosa[J]. Journal of Bengbu Medical College, 2013, 37(10): 1328-1330.
    Citation: ZHANG Lun-jun, DING Xiao-lin, GUO Pu, ZHONG Zheng-rong, SHEN Ji-long. The antibiotic-resistance and its gene analysis of extended-spectrum -lactamases in imipenem-resistant Pseudomonas aeruginosa[J]. Journal of Bengbu Medical College, 2013, 37(10): 1328-1330.

    耐亚胺培南铜绿假单胞菌的耐药性及超广谱-内酰胺酶的基因分析

    The antibiotic-resistance and its gene analysis of extended-spectrum -lactamases in imipenem-resistant Pseudomonas aeruginosa

    • 摘要: 目的:探讨临床分离铜绿假单胞菌的药敏谱及其产超广谱-内酰胺酶(ESBLs)的情况、基因分布和流行特性。方法:分析208株铜绿假单胞菌对16种抗生素的药敏谱,筛选耐亚胺培南铜绿假单胞菌,采用PCr检测ESBLs基因(TEM、SHV、OXA-10、OXA-2、PEr、VEB)。结果:53株对亚胺培南耐药,耐药率为25.48%(53/208),且对头孢替坦(CTE)、头孢唑林(CZ)、氨苄西林/舒巴坦(AMS)、呋喃妥因(FTN)等耐药率较高;53株亚胺培南耐药菌经PCr检测,产ESBLs菌株23株,其中TEM型16株,OXA-10型4株,PEr型3株,未检测出OXA-2和SHV型。结论:耐亚胺培南铜绿假单胞菌中产ESBLs菌株的基因型主要为TEM型,且为多重耐药,临床应结合药敏谱,合理使用抗生素。

       

      Abstract: Objective: To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa and extended-spectrum -lactamase (ESBLs) producing condition, gene distribution and epidemiological characteristics in clinic. Methods: The antimicrobial susceptibility was detected with the Vitek-2 compact automatic bacteria identification system, 16 antibiotics for 208 Pseudomonas aeruginosa isolated. The imipenem-resistant Pseudomonas aeruginosa were selected. ESBLs genes produced by imipenem resistance were detected by PCr, the ESBLs genes contain TEM,SHV,OXA-10,OXA-2,PEr and VEB. results: In the 208 isolates,53 strains of Pseudomonas aeruginosa were resistant to imipenem, the drug resistance rate was 25. 48%. The isolates had a high rate of drug-resistance against CTE,CZ,AMS and FTN; The PCr results showed that in the 53 imipenem-resistant Pseudomonas aeruginosa,ESBLs gene were produced in 23 strains, 16 strains were TEM,4 for OXA-10 and 3 for PEr gene; OXA-2,SHV was not detected. Conclusions: TEM of Extended-spectrum-lactamase producing in imipenem-resistant Pseudomonas aeruginosa is the main gene in our hospital,most of them are multi-drug-resistance,detecting the antimicrobial susceptibility and using antibiotics rationally is essential in clinic.

       

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