Abstract: Objective: To investigate the infection status of Helicobacter pylori(Hp) among healthy population in Bengbu City. Methods: Immune colloidal gold assay was used to detect Hp urease antibody in serum among 2 283 cases of healthy people in Bengbu city. Results: The overall Hp infection rate was 16. 1% in total 2 283 cases. Among them, the infection rate for male was 16. 9%, and for female was 14. 2%, it was no statistical difference among male and female(P ＞ 0. 05) . There were statistical significances about Hp infection rate among different ages in male(P ＜ 0. 01) , but no statistical significance among different ages in female(P ＞ 0. 05) . The infection rates of male in 50-59 age group and above 70 age group were higher than those of female groups and was statistically significant(P ＜ 0. 05) . Conclusions: The infection rate of Hp was high in healthy population in Bengbu. Hence, Hp detection should be routinely conducted in physical examination. The early precaution will be useful for early cure and prevent relevant diseases.