Abstract: Objective: To explore the significance of high-risk human papilloma virus( HR-HPV) detection in cervical cancer screening. Methods: Two thousand four hundred and forty-two patients were detected using liquid base cytology( TCT),2 147 patients were detected by HPV hybrid capture method of the second generation( HPV-HC Ⅱ) or nucleic acid molecular hybridization technique,TCT and HPV testing of which 783 patients were simultaneously detected. HR-HPV testing in 723 cases with normal or abnormal cytology were positive,the patients with normal cytology,negative HR-HPV and clinical highly suspected lesions were treated with multipoint biopsy under colposcope and cervical electric ring resection( LEEP),these data combined with pathological results were analyzed. Results: Amony samples with TCT and HPV testing,the HR-HPV positive rates in normal or inflammation TCT,ASC-US,lowgrade squamous intraepithelial lesion and high-grade squmous intraepithelial lesion were 22. 39%,48. 09%,74. 47% and 88. 46%, respectively,the difference of which was statistical significance( P 0. 01). The HR-HPV positive rate increased significantly with the increasimg of cytology diagnosis level. LEEP pathological results showed the HR-HPV positive rates in normal or inflammation tissue, cervical intraepithelial neoplasia( CIN) 1,CIN 2,CIN 3,squamous cell carcinoma and condyloma were 23. 72%,83. 87%,85. 19%, 88. 10%,92. 86% and 72. 37%,respectively,the difference of which was statistical significant( P 0. 01). Conclusions: The continuous HR-HPV positive is related to the evolution of cervical lesions,the HR-HPV detection has important significant in screening cervical cancer.