Abstract: Objective: To reform the professional course teaching of preventive medicine undergraduate students for meeting the need of public health service development. Methods: Forty grade 3rd undergraduate students from preventive medicine were selected by stratified sampling method according to average scores,and divided into participatory and control group( 20 students in each group). The control group were taught by routine mode. Bilingual teaching was applied in participatory group,participatory forms included playing sketch,drawing,designing game and making model. Professional knowledge,attitude and behavior of two groups were compared. Results: The score difference of professional knowledge between two groups has no statistical significance( P 0. 05). The scores of attitude and behavior in participatory group were significantly higher than those in control group( P 0. 01). The teaching and social effects of participatory group were better than those in control group. Conclusions: Participatory teaching method can enrich practical education and improve the learning activity of professional knowledge,which can provide a model for related educators.