反复吸入2.3%七氟烷对幼年大鼠学习记忆与乙酰胆碱酯酶活性的影响

    The effect of repeated inhaling of 2.3% sevoflurane on learning and memory and the activity of acetylchalinesterase in young rats

    • 摘要: 目的:观察幼年大鼠反复吸入2.3%七氟烷对学习记忆能力的影响。方法:将健康雄性SD大鼠60只随机均分为6组,Con1组、Con3组、Con5组:每天同一时间吸氧1 h,流量0.5 L/min,分别吸1、3、5 d;Sev1组、Sev3组、Sev5组:每天同一时间吸入2.3%七氟烷和流量0.5 L/min氧气的混合气体1 h,分别吸1、3、5 d。末次给药7 d后,进行水迷宫实验,观察大鼠达标所需训练次数、逃避潜伏期及进入盲端的次数,行为学实验后测定乙酰胆碱酯酶活性。结果:与Con1、Con3、Con5组比较,Sev1、Sev3、Sev5组大鼠达标所需训练次数、逃避潜伏期、进入盲端次数以及乙酰胆碱酯酶活性差异均无统计学意义(P0.05)。结论:持续5 d,每天吸入2.3%七氟烷1 h,末次给药7 d后对于幼年大鼠空间辨别学习记忆功能无明显影响。

       

      Abstract: Objective: To investigate the effects of repeated inhaling of 2. 3% sevoflurane on learning and memory in young rats. Methods: Sixty SD male rats were randomly divided into Con1,Con3,Con5,Sev1,Sev3 and Sev5 group( 10 rats each group). The group Con1,Con3 and Con5 inhaled oxygen( 0. 5 L / min) 1 hour every day for 1,3 and 5 days,respectively. The group Sev1,Sev3 and Sev5 inhaled the mixed gas of 2. 3% sevoflurane and oxygen( 0. 5 L / min) 1 hour every day for 1,3 and 5 days,respectively. Water maze test in all groups were implemented 7 days later after the last administration. The training number of rats with requiring standard,escaping latency period,the number of entering blind end and activity of acetylcholinesterase after behavioral experiment were observed. The effects of repeated inhaling of 2. 3% sevoflurane on learning and memory in young rats were observed after 7 days. Results: The differences of the training number of rats with requiring standard,escaping latency period,the number of entering blind end and activity of acetylcholinesterase in all groups were not statistical significance( P 0. 05). Conclusions: The repeated inhaling 2. 3% sevoflurane 1 hour every day for 5 days in young rats have no effects on their learning and memory founction after 7 days.

       

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