Abstract: Objective:To explore the technical parameters of magnetic resonance diffusion tensor imaging(DTI) in corpus callosum. Methods:The DTI of the sagittal plane were detected in 30 nomarl brains.The effects of b value,slice thickness/interlamellar spacing and diffusion sensitive gradient direction on fractional anisotropy(FA) image quality were compared.Results:Different parameters had different effects on FA image quality.The quality of FA image was best when b value was middle(b = 1000 s/mm²),FA image noise was large at the high b value(b = 1500 s/mm²),and the contrast of FA image was poor at the low b value(b = 500 s/mm²).The signal to noise ratio was high with slice thickness/interlamellar spacing increasing,the subtle structure was easy to miss.The differences of FA image qualities at 13 and 25 diffusion sensitive gradient directions statistical significance (P 0.05).The FA image quality at 6 diffusion sensitive gradient direction was worse hadno.Conclusions:The 1000 s/mm² of b value,5 mm/0 mm of slice thickness and 15 diffusion sensitive gradient directions are suitable for DTI of corpus callosum in the sagittal head in clinic.