蜕膜自然杀伤细胞的表型及功能变化
The changes of decidual natural killer cells phenotype and function
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摘要: 目的:通过比较蜕膜自然杀伤细胞(dNK细胞)与外周血NK细胞(pNK细胞)的表型及功能变化,探讨dNK细胞特殊的表型和功能与母胎界面免疫耐受调节机制的关系。方法:收集晚孕胎膜组织及外周血,流式细胞术检测dNK细胞与pNK细胞表型的差异;免疫磁珠分选dNK细胞与pNK细胞,乳酸脱氢酶法测定其对K562的自然杀伤活性。结果:dNK细胞表面CD16的表达明显低于pNK细胞(P0.01),NKG2A的表达明显高于pNK细胞(P0.01),NKG2D的表达低于pNK细胞(P0.05);dNK细胞对K562的自然杀伤活性明显低于pNK细胞(P0.01)。结论:dNK细胞特殊的表型和功能可能是维持母胎界面免疫耐受的重要因素。Abstract: Objective:To compare the phenotype and functional changes between decidual natural killer(dNK) cells and peripheral natural killer (pNK) cells,and investigate the relationship of dNK cells specific phenotype and the immunotolerance regulated mechanism at fetal-maternal interface.Methods:The phenotype of dNK cells and pNK cells was analyzed with flow cytometry.dNK cells and pNK cells were separated by MACS,lactate dehydrogenase was used to determine the cytotoxic activity on K562 cells.Results:CD16 expression on dNK cells was significantly lower than on pNK cells(P 0.01).NKG2A expression on dNK cells was higher than that on pNK cells(P 0.01),NKG2D expression on dNK cells was lower than on pNK cells(P 0.05).The cytotoxic activity of dNK cells on K562 cells was significantly lower than that of pNK cells.Conclusions:The specific phenotype and function of dNK cells may be an important factors for the immunotolerance maintenance at fetal-maternal interface during pregnancy.
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