再生障碍性贫血患者外周血单个核细胞T-bet表达及其意义

Expression of T-bet in peripheral blood mononuclear cells of patients with aplastic anemia and its significance

摘要: 目的:探讨外周血单个核细胞T-bet表达与再生障碍性贫血(aplastic anemia,AA)发病之间的关系。方法:半定量RT-PCR法检测17例初诊AA患者和20名健康者外周血单个核细胞(PBMC)T-bet的表达,ELISA法检测血浆干扰素-γ(IFN-γ)水平。结果:初诊AA患者PBMC中T-bet表达和血浆IFN-γ水平均显著高于正常对照组(P<0.01),两者呈正相关关系(P<0.05)。结论:T-bet和IFN-γ表达的增高在AA的免疫病理学中可能起着重要作用。

Abstract: Objective: To explore the relationship between the expression of T-bet gene in peripheral blood mononuclear cells(PBMC) and pathogenesis of aplastic anemia(AA).Methods: T-bet mRNA expression in PBMC was semi-quantitatively analyzed by the reverse transcription PCR(RT-PCR) and the serum level of interferon-γ(IFN-γ) was detected by ELISA in total 37 cases,including 17 cases of the firstly diagnostic patients with AA,and 20 healthy individuals as controls.Results: As compared with those in normal control group,the expression of T-bet in PBMC and the serum level of IFN-γ in the firstly diagnostic patients with AA increased with significant difference(P<0.01),the correlativity of them appeared positive(P<0.05).Conclusions: The increased expression of both T-bet and IFN-γ may play an important role in immunopathology of AA.