Abstract: Objective:To establish a simple and convenient method of preparing and isolating soluble protein from Plasmodium berghei(P.berghei).Methods:SD rats were infected by mouse blood containing P.berghei,and then the rat peripheral blood was collected at the P.berghei-infected late stage.Furthermore the P.berghei-infected ＲBC were isolated by 25% arabic gum.The soluble protein was prepared from P.berghei by ultrasonication.The soluble protein was analyzed by SDS-PAGE,and compared with soluble protein from P.berghei-infected mice.Ｒesults:The large-scale yield of P.berghei can be prepared from P.berghei-infected rats,and the enough soluble protein can be obtained from P.berghei by ultrasonication.The proportion of each protein in soluble protein of P.berghei from infected rats is as the same as the protein from infected mice.Conclusions:The method of separating P.berghei from infected rats is a kind of simple and convenient way.The large-scale quantity of soluble protein antigen of P.berghei can be obtained by ultrasonication.