亚甲基四氢叶酸还原酶基因多态性与2型糖尿病的相关性
The related study between methylenetetrahydrofolate reductase gene polymorphism and type 2 diabetes mellitus
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摘要: 目的:探讨亚甲基四氢叶酸还原酶(MTHFR)基因C677T和A1298C多态性与2型糖尿病(T2DM)的关系。方法:选取106例T2DM患者(病例组)和109名健康对照个体(对照组),采用聚合酶链反应-限制性片段多态性分别检测C677T和A1298C多态位点等位基因频率和基因型并统计分析。结果:2组MTHFR基因C677T和A1298C位点基因型和等位基因频率比较差异均无统计学意义(P》0.05),T2DM发病中2个突变位点之间没有协同作用(P》0.05)。结论:MTHFR基因C677T和A1298C多态位点与T2DM的易感性无明显的相关性。Abstract: Objective: To examine the relationship between methylenetetrahydrofolate reductase(MTHFR) gene C677T and A1298C polymorphisms and type 2 diabetes mellitus(T2DM). Methods: MTHFR gene C677T and A1298C polymorphic sites were assayed in 106 T2DM and 109 healthy subjects by polymerase chain reaction-restriction fragment length polymorphism, and their allele frequency and genotype were statistically analyzed.Results: There was no statistical significance in allele frequency and genotype of MTHFR gene C677T and A1298C between the patients and healthy subjects(P > 0. 05), and no synergistic reaction between the MTHFR C677T andA1298C polymorphisms on the risk of T2DM(P > 0. 05). Conclusions: MTHFR gene C677T and A1298C polymorphism is not a risk facObjective: Tor for patients with type 2 diabetes.