骨髓自然杀伤细胞水平对急性髓细胞性白血病患者预后的影响
The effect of natural killer cells level in bone marrow on the prognosis of patients with acute myeloid leukemia
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摘要: 目的：探讨骨髓自然杀伤(natural killer,NK)细胞水平对急性髓细胞性白血病(acute myeloid leukemia,AML)患者预后的影响。方法：研究分成AML初治组40例,缓解组20例,正常对照组20例。均抽取骨髓液2 ml,采用直接免疫荧光标记法对骨髓有核细胞进行单克隆抗体(CD16+/CD56+)标记,应用流式细胞仪进行NK细胞数量的检测。结果:初治组患者骨髓NK细胞表达水平较缓解组显著降低(P0.01)。缓解组患者骨髓NK细胞表达水平仍低于对照组(P0.05)。不同骨髓NK细胞表达水平间原始细胞数量和AML患者疗效差异均有统计学意义(P0.01和P0.05)。结论：骨髓NK细胞表达水平可以作为AML的预后指标,亦有助于指导治疗。Abstract: Objective: To explore the effects of natural killer (NK) cells in bone marrow on the prognosis of patients with acute myeloid leukemia(AML). Methods: Sixty patients with AML were divided into original treating group(40 cases) and remission group (20 cases), 20 health people were served as control group． The CD16 + /CD56 + of monoclonal antibodies in bone marrow nucleatedcells of three groups were detected by direct immunofluorescence labeling method， the levels of NK cells were determined with flowcytometry． Results: The levels of NK in original treating group were significantly lower than those in remission group(P ＜ 0． 01), the levels of NK in remission group were significantly lower than those in normal group(P ＜ 0． 05). The original cell number in different NK cell levels and the differences of curative effect in AML patients were statistically significant(P ＜ 0． 01 and P ＜ 0． 05). Conclusions:The level of NK cells in bone marrow is a prognostic index of AML and can help to guide treatment．
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