张立功, 贾建光, 金鑫, 谢波, 承泽农, 钱军. PTEN与P-AKT在胃癌中的表达及意义[J]. 蚌埠医学院学报, 2014, 38(3): 291-294.
    引用本文: 张立功, 贾建光, 金鑫, 谢波, 承泽农, 钱军. PTEN与P-AKT在胃癌中的表达及意义[J]. 蚌埠医学院学报, 2014, 38(3): 291-294.
    ZHANG Ligong, JIA Jianguang, JIN Xin, XIE Bo, CHENG Zenong, QIAN Jun. The expression of PTEN and P-AKT in gastric carcinoma and its significance[J]. Journal of Bengbu Medical College, 2014, 38(3): 291-294.
    Citation: ZHANG Ligong, JIA Jianguang, JIN Xin, XIE Bo, CHENG Zenong, QIAN Jun. The expression of PTEN and P-AKT in gastric carcinoma and its significance[J]. Journal of Bengbu Medical College, 2014, 38(3): 291-294.

    PTEN与P-AKT在胃癌中的表达及意义

    The expression of PTEN and P-AKT in gastric carcinoma and its significance

    • 摘要: 目的:检测人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)与磷酸化丝氨酸/苏氨酸蛋白激酶B(P-AKT)在胃癌和癌旁组织中的表达,探讨它们在胃癌发生、发展中的作用。方法:应用免疫组织化学EliVision法,检测68例胃癌及其癌旁组织中PTEN和P-AKT蛋白表达水平,同时对68例胃癌和癌旁组织采用反转录-聚合酶链反应检测其中PTEN mRNA的表达。结果:PTEN蛋白在胃癌组织中表达低于癌旁组织(P0.01),P-AKT蛋白在胃癌组织中表达高于癌旁组织(P0.01),两者呈负相关关系(P0.05)。胃癌组织中PTEN蛋白的表达在性别和年龄间差异均无统计学意义(P0.05),但在分化程度、肿瘤大小、临床分期和淋巴结转移表达差异均有统计意义(P0.01)。胃癌组织中P-AKT蛋白表达在性别、年龄和肿瘤大小间差异均无统计学意义(P0.05),但在分化程度、临床分期和淋巴结转移间表达差异均有统计学意义(P0.05)。胃癌组织PTEN mRNA的半定量表达明显低于癌旁组织PTEN mRNA的半定量表达(P0.01)。结论:PTEN基因在胃癌组织中低表达,癌旁组织蛋白表达也明显高于胃癌组织,P-AKT蛋白在胃癌组织中的表达高于癌旁组织,两者在胃癌中的表达呈负相关。

       

      Abstract: Objective: To detect the expressions of phosphatase and tension protein homologous gene ( PTEN) in human with chromosome 10 deletion and phosphorylated AKT(P-AKT) in gastric carcinoma and adjacent tissue,and explore their roles in the development of gastric carcinoma. Methods:The mRNA and protein expression levels of PTEN and P-AKT in 68 gastric carcinoma and adjacent gastric tissue specimens were detected using immunohistochemistry EliVision and reverse transcription polymerase chain reaction(RT-PCR),respectively. Results:The PTEN and P-AKT protein levels in gastric carcinoma tissue specimen were significantly lower and higher than those in the adjacent tissue,respectively(P 0. 01),which was negative correlation(P 0. 05). No correlation between the expression of PTEN protein in gastric carcinoma and patients age and sexuality was found(P >0. 05),but the its expression was related to the differentiation degree,size,clinical stage and lymph node metastasis of tumor(P 0. 01). No correlation between the expression of P-AKT protein in gastric carcinoma and patients age and sexuality and tumor size was found(P0. 05),but the its expression was related to the differentiation degree,clinical stage and lymph node metastasis of tumor(P 0. 05). The PTEN mRNA level in gastric carcinoma was significantly lower than that in adjacent tissue(P 0. 01). Conclusions:The mRNA expression of PTEN gene in gastric carcinoma is lower,its protein expression in adjacent tissueis higher than that in gastric carcinoma. The expression of P- AKT protein in gastric carcinoma is higher than that in adjacent tissue. There is negatively correlated between the protein expressions of PTEN and P-AKT in gastric carcinoma.

       

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