Abstract: Objective: To detect the expressions of phosphatase and tension protein homologous gene ( PTEN) in human with chromosome 10 deletion and phosphorylated AKT(P-AKT) in gastric carcinoma and adjacent tissue，and explore their roles in the development of gastric carcinoma． Methods:The mRNA and protein expression levels of PTEN and P-AKT in 68 gastric carcinoma and adjacent gastric tissue specimens were detected using immunohistochemistry EliVision and reverse transcription polymerase chain reaction(RT-PCR)，respectively． Results:The PTEN and P-AKT protein levels in gastric carcinoma tissue specimen were significantly lower and higher than those in the adjacent tissue，respectively(P 0． 01)，which was negative correlation(P 0． 05)． No correlation between the expression of PTEN protein in gastric carcinoma and patients age and sexuality was found(P ＞0． 05)，but the its expression was related to the differentiation degree，size，clinical stage and lymph node metastasis of tumor(P 0． 01)． No correlation between the expression of P-AKT protein in gastric carcinoma and patients age and sexuality and tumor size was found(P0． 05)，but the its expression was related to the differentiation degree，clinical stage and lymph node metastasis of tumor(P 0． 05)． The PTEN mRNA level in gastric carcinoma was significantly lower than that in adjacent tissue(P 0． 01)． Conclusions:The mRNA expression of PTEN gene in gastric carcinoma is lower，its protein expression in adjacent tissueis higher than that in gastric carcinoma． The expression of P- AKT protein in gastric carcinoma is higher than that in adjacent tissue． There is negatively correlated between the protein expressions of PTEN and P-AKT in gastric carcinoma．