腺病毒介导的生长抑制基因4对ECV304细胞体外生长及分泌血管内皮生长因子的影响

张海峰; 盛伟华; 缪竞诚; 陈卫东; 杨吉成

腺病毒介导的生长抑制基因4对ECV304细胞体外生长及分泌血管内皮生长因子的影响

The effect of adenovirus-mediated inhibitor of growth family member 4 on growth and vascular endothelial growth factor secretion of ECV304 cells

摘要

摘要: 目的: 检测腺病毒介导的生长抑制基因4(adenovirus-mediated inhibitor of growth family member 4,Ad-ING4)感染后ECV304细胞体外生长及分泌血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响,探讨ING4抑制肿瘤生长的可能机制。方法: 构建ING4腺病毒载体,用MTT法检测Ad-ING4感染后ECV304细胞体外生长影响,ELISA检测VEGF的分泌情况。结果: Ad-ING4感染后第3天和第4天ECV304细胞体外生长均受到抑制(P < 0.01),第4天时抑制率达63.6%,VEGF分泌受到抑制。结论: ING4能抑制ECV304细胞生长及VEGF分泌。Ad-ING4能抑制血管内皮细胞生长和血管的生成,从而可抑制体内肿瘤的生长。

Abstract: Objective: To interpret the possible anti-tumor mechanism of adenovirus-mediated inhibitor of growth family member 4 (Ad-ING4) by testing the growth and vascular endothelial growth factor(VEGF) expression of ECV304 cells infected by Ad-ING4. Methods: Adenovirus vector of ING4 was constructed,then the growth of ECV304 cells was detected by MTT,and the VEGF expression of ECV304 cells infected by Ad-ING4 was detected by ELISA. Results: The VEGF expression of ECV304 cells infected by Ad-ING4 decreased,and the growth of ECV304 was inhibited,and the inhibition ratio was 63.6% on the fourth day. Conclusions: ING4 could down regulate the expression of VEGF,and inhibit the growth of ECV304,and ING4 maybe through this way affect growth,and tumor angiogenesis of lung cancer.

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